Husbandry of Zebrafish, Danio Rerio, and the Cortisol Stress Response
| Autor: | Aurora Campo, Alexandra Tsalafouta, Michail Pavlidis, Athanasios Samaras, Gregoris Skouradakis, Antonia Theodoridi, Konstantinos Barsakis, Nikoletta Digka |
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| Rok vydání: | 2013 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Hydrocortisone Danio Color Fight-or-flight response 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Stocking Aquaculture Stress Physiological Water Quality Internal medicine medicine Animals 14. Life underwater Animal Husbandry Social Behavior Zebrafish 030304 developmental biology Population Density 0303 health sciences biology business.industry Animal husbandry biology.organism_classification Trunk Crowding Endocrinology Animal Science and Zoology business 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Zebrafish. 10:524-531 |
| ISSN: | 1557-8542 1545-8547 |
| DOI: | 10.1089/zeb.2012.0819 |
| Popis: | The effect of common husbandry conditions (crowding, social environment, water quality, handling, and background color) on the cortisol stress response in adult zebrafish, Danio rerio, was investigated to check the usefulness of zebrafish as a model organism in aquaculture research. In addition, a noninvasive methodology for assessing stress was evaluated. Zebrafish showed a fast cortisol response with high values at 30 min that returned to basal levels within 2 h of poststress. There was a significant positive correlation between trunk cortisol concentrations and the free water cortisol rate (r(2)=0.829-0.850, p0.001), indicating that measurement of the water-borne cortisol release rate may serve as a noninvasive and reliable stress indicator at the population level. Crowding resulted in 13- to 21-fold greater mean trunk cortisol concentrations compared with controls. However, even at low stocking density (2-5 fish/L), the maintenance cost was higher than the one at higher densities (10 fish/L) due to the formation of dominance hierarchies. The background color affected trunk cortisol concentrations, with fish exposed to brighter backgrounds (green and white) showing 3- to 8-fold greater mean trunk cortisol concentrations than fish exposed to a black background or transparent aquaria. Fish exposed to high stocking densities for 2 h or 5 days had similar high mean trunk cortisol levels, indicating that exposure of fish for the period of 2 h to a specific stressor may represent a chronic situation in zebrafish. It is concluded that adult laboratory zebrafish had a preference for a transparent or black background aquarium, at a number of 10 individuals per 2 L of available water volume, to express their normal behavior and avoid increased cortisol stress reaction. |
| Databáze: | OpenAIRE |
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