Characterization of two Austrian porcine reproductive and respiratory syndrome virus (PRRSV) field isolates reveals relationship to East Asian strains
| Autor: | Till Rümenapf, Christiane Riedel, Bettina Jansko, Hanna C. Koinig, Benjamin Lamp, Isabel Hennig-Pauka, Leonie Zieglowski, Georg Mößlacher, Leonie J. Sinn |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Gene Expression Regulation
Viral Male 0301 basic medicine Swine [SDV]Life Sciences [q-bio] Molecular Sequence Data Porcine Reproductive and Respiratory Syndrome Biology Simian Genome Disease Outbreaks Viral Proteins 03 medical and health sciences Macrophages Alveolar Genotype Bacteriology Animals Porcine respiratory and reproductive syndrome virus Amino Acid Sequence Viremia Cells Cultured Phylogeny 2. Zero hunger Virulence General Veterinary Phylogenetic tree Asia Eastern Strain (biology) Outbreak biology.organism_classification Porcine reproductive and respiratory syndrome virus veterinary(all) Virology Virus Shedding 3. Good health 030104 developmental biology Austria Female Research Article |
| Zdroj: | Veterinary Research Veterinary Research, BioMed Central, 2016, 47 (1), pp.17. ⟨10.1186/s13567-015-0293-x⟩ |
| ISSN: | 0928-4249 1297-9716 |
| DOI: | 10.1186/s13567-015-0293-x⟩ |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) causes major problems for the swine industry worldwide. Due to Austria’s central location in Europe, a large number of animals are transported through the country. However, little is known about current PRRSV strains and epidemiology. We determined full-length genome sequences of two Austrian field isolates (AUT13-883 and AUT14-440) from recent PRRSV outbreaks and of a related German isolate (GER09-613). Phylogenetic analysis revealed that the strains belong to European genotype 1 subtype 1 and form a cluster together with a South Korean strain. Remarkably, AUT14-440 infected the simian cell line MARC-145 without prior adaptation. In addition, this isolate showed exceptional deletions in nonstructural protein 2, in the overlapping region of glycoprotein 3 and 4 and in the 3′ untranslated region. Both Austrian isolates caused similar lung lesions but only pigs infected with AUT14-440 developed clear clinical signs of infection. Taken together, the genetic and biological characterization of two novel Austrian PRRSV field isolates revealed similarities to East Asian strains. This stresses the necessity for a more detailed analysis of current PRRSV strains in Europe beyond the determination of short ORF5 and ORF7 sequences. Electronic supplementary material The online version of this article (doi:10.1186/s13567-015-0293-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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