Scanning electron microscopy of the surface epithelium of the bovine endometrium
| Autor: | M.C. Lucy, Eleanore V O'Neil, Thomas E. Spencer, F.G. Kumro, L.A. Ciernia, J.G.N. Moraes |
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| Rok vydání: | 2020 |
| Předmět: |
Cell type
Morphology (linguistics) Cell Estrous Cycle Luteal Phase Endometrium Epithelium Article Andrology 03 medical and health sciences Corpus Luteum Pregnancy Genetics medicine Animals 030304 developmental biology Estrous cycle 0303 health sciences Microvilli urogenital system Chemistry Cilium 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science medicine.anatomical_structure Microscopy Electron Scanning Cattle Female Animal Science and Zoology Corpus luteum Food Science |
| Zdroj: | J Dairy Sci |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2020-18852 |
| Popis: | The surface epithelium of the bovine endometrium comprises at least 2 cell types (ciliated cells and secretory cells with microvilli), but their distribution and morphological changes over the estrous cycle are poorly understood. The objective was to quantify the number of ciliated cells and assess morphological changes in secretory cells on the uterine surface epithelium during the estrous cycle. Caruncular endometrium (CAR) and intercaruncular endometrium (ICAR) samples were collected from the uterine body, the horn ipsilateral to the corpus luteum or dominant follicle (H-CL/DF), and the horn contralateral to the corpus luteum or dominant follicle (H-NCL/NDF) from heifers following slaughter on d 0 (estrus; n = 5) or d 14 (mid-luteal phase; n = 5) of the estrous cycle. Samples were prepared for scanning electron microscopy at 1,000× magnification. Four to 10 fields (256 × 225 µm) for each sample were examined (n = 567 images). The number of ciliated cells was counted and the surface was scored for the morphology of the secretory cells (0 = absence of microvilli on surface; 3 = 100% of surface covered with microvilli). Ciliated cells were present in both the CAR and ICAR regions. The number of ciliated cells per field increased from d 0 to 14 in CAR and decreased from d 0 to14 in ICAR. The scanning electron microscopy revealed a general lack of uniformity in the lawn of microvilli on the surface of the endometrium. Based on the scores, approximately 25% of the fields had a surface that was |
| Databáze: | OpenAIRE |
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