Piglet weight gain during the first two weeks of lactation influences the immune system development
| Autor: | Frédéric Beaudoin, Nathalie Bissonnette, Karoline Lauzon, Frédéric Guay, L. Lo Verso, Karine Deschene, Mylène Blais, Martin Lessard |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Litter (animal) Swine animal diseases Immunology Population Gene Expression Ileum Enzyme-Linked Immunosorbent Assay Biology Real-Time Polymerase Chain Reaction Weight Gain Peripheral blood mononuclear cell Andrology 03 medical and health sciences fluids and secretions Immune system Lactation Gene expression medicine Animals education education.field_of_study integumentary system General Veterinary Microarray Analysis 030104 developmental biology medicine.anatomical_structure Immune System Leukocytes Mononuclear Cytokines Female medicine.symptom Weight gain |
| Zdroj: | Veterinary immunology and immunopathology. 206 |
| ISSN: | 1873-2534 |
| Popis: | The aim of this study was to investigate the effect of the piglet growth during the first week of life on ileal expression of genes and on development of the immune system. Eight litters adjusted to 12 piglets were used. Within each litter, the piglet that showed the lowest weight gain (LWG; n = 8) and the one that showed the highest weight gain (HWG; n = 8) in their first week of life were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated on days 8 and 16 to characterize cellular population profiles and to assess ex-vivo secretion of interleukin-10 (IL-10), IL-6 and tumor necrosis factor-α (TNF-α). On day 16, piglets were euthanized and ileum samples were collected to extract RNA for microarray analysis and gene expression by qPCR. As expected, growth performance of LWG piglet was impaired compared to HWG piglets (P 0.05). From day 8 to 16, the percentage of CD21 |
| Databáze: | OpenAIRE |
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