Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4

Autor: Adelaide Braga, Daniela Gomes, João Rainha, Beatriz B. Cardoso, Cláudia Amorim, Sara C. Silvério, María Fernández-Lobato, Joana L. Rodrigues, Lígia R. Rodrigues
Přispěvatelé: Fundação para a Ciência e a Tecnologia (Portugal), Braga, Adelaide, Gomes, Daniela, Rainha, João, Cardoso, Beatriz B., Amorim, Cláudia, Silvério, Sara C., Fernández-Lobato, María, Rodrigues, Joana L., Rodrigues, Lígia R.
Rok vydání: 2022
Předmět:
Zdroj: Applied Microbiology and Biotechnology. 106:4617-4626
ISSN: 1432-0614
0175-7598
DOI: 10.1007/s00253-022-12037-3
Popis: 10 pags., 2 figs., 1 tab.
Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4 ± 1.6 g L-1 of FOS, with a productivity of 1.53 ± 0.03 g L-1 h-1 and a yield of 0.31 ± 0.03 gFOS gsucrose-1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. KEY POINTS: • Zymomonas mobilis was engineered to produce FOS in a one-step fermentation process. • Mutant strains produced FOS mixtures with high concentration of 6-kestose. • A new route to produce tailor-made FOS mixtures was presented.
The study received financial support from Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and the project FoSynBio—Synthetic biology approaches to design and construct microbial cell factories for the production of fructooligosaccharides (POCI-01–0145-FEDER-029549).
Databáze: OpenAIRE
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