Performance of Xpert MTB/RIF in comparison with light-emitting diode-fluorescence microscopy and culture for detecting tuberculosis in pulmonary and extrapulmonary specimens in Bamako, Mali
| Autor: | Baya Bocar, Dembele Bindongo Price Polycarpe, Kone Bourahima, Somboro Anou Moise, Kodio Ousmane, Drame Hawa Baye, Diallo Fatimata, Traore Aissata Boubakar, Coulibaly Gagni, Belson Michael, Degoga Boureima, Doumbia Seydou, Sanogo Moumine, D.S. Diallo Souleymane, Togo Antieme Combo Georges, Maiga Mamoudou, D.B. Diarra Bassirou, Sarro Yeya Dit Sadio, Coulibaly Cheick Abou, Tolofoudie Mohamed, Bane Sidy, Traore Aissata, Somboro Amadou, I Maiga Ibrahima |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Tuberculosis lcsh:QR1-502 Molecular Technique Sensitivity and Specificity Gastroenterology lcsh:Microbiology Smear microscopy Internal medicine medicine Humans bamako Tuberculosis Pulmonary mali business.industry Extrapulmonary tuberculosis Sputum Mycobacterium tuberculosis Gold standard (test) medicine.disease light-emitting diode fluorescent microscopy culture xpert mtb/rif Infectious Diseases Microscopy Fluorescence Mycobacterium culture Rifampin medicine.symptom business Previously treated |
| Zdroj: | International Journal of Mycobacteriology, Vol 9, Iss 4, Pp 397-404 (2020) |
| ISSN: | 2212-5531 |
| Popis: | Background: The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB diagnosis and detection of resistance. Our current study was undertaken to compare the diagnostic performance of Xpert MTB/RIF assay to auramine staining-based light-emitting diode-Fluorescence Microscopy (LED-FM) considering culture as the gold standard method for pulmonary and extrapulmonary TB. Method: Pulmonary and extrapulmonary specimens of suspected TB patients were examined in this study. From January 2016 to June 2019, sputum, urine, superficial swabs, gastric aspirates, and pleural infusion specimens were collected from new and previously treated TB individuals. Specimens were examined using Xpert MTB/RIF, LED-FM, and Mycobacterium culture techniques to evaluate their performance. Results: A total of 697 suspected TB samples were included in this analysis, and of these, 469 (67.29%) were positive for all three used methods. The overall sensitivities, specificities, and positive and negative predictive values were 99.6%, 62.0%, 88.4%, and 98.2% for Xpert MTB/RIF and 88.0%, 95.6%, 99.0%, and 60.7% for LED-FM, respectively, compared to culture method. Conclusion: The sensitivity of Xpert MTB/RIF assay was observed to be higher than the LED-FM method, thus suggesting this molecular technique as a promising tool for the diagnosis of pulmonary and extrapulmonary TB, which will help in the management of TB infections in developing countries such as Mali. |
| Databáze: | OpenAIRE |
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