Aptamer-Assisted Protein Orientation on Silver Magnetic Nanoparticles: Application to Sensitive Leukocyte Cell-Derived Chemotaxin 2 Surface Plasmon Resonance Sensors

Autor:	Han Zhu, Yongkai Lu, Junjie Xia, Yawen Liu, Jie Chen, Jaebeom Lee, Kwangnak Koh, Hongxia Chen
Rok vydání:	2022
Předmět:	Silver Chemotactic Factors Leukocytes Surface Plasmon Resonance Magnetite Nanoparticles Analytical Chemistry
Zdroj:	Analytical Chemistry. 94:2109-2118
ISSN:	1520-6882 0003-2700
DOI:	10.1021/acs.analchem.1c04448
Popis:	Leukocyte cell-derived chemotaxin 2 (LECT2) has been proved to be a potential biomarker for the diagnosis of liver fibrosis. In this work, a sensitive surface plasmon resonance (SPR) assay for LECT2 analysis was developed. Tyrosine kinase with immune globulin-like and epidermal growth factor-like domains 1 (Tie1) is an orphan receptor of LECT2 with a C-terminal Fc tag, which is far away from the LECT2 binding sites. The Fc aptamer was intentionally used to capture the Tie1 through its Fc tag, connecting with Fe
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb031fcc359e46be9ed27edaa1a0d1e7 https://doi.org/10.1021/acs.analchem.1c04448 Zobrazit plný text záznamu