IFNγ induces JAK1/STAT1/p65 NFκB-dependent interleukin-8 expression in ovarian cancer cells, resulting in their increased migration
| Autor: | Bijaya Gaire, Daniel DeLeon, Sveta Padmanabhan, Mohammad M. Uddin, Yue Zou, Ivana Vancurova |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ovarian Neoplasms
Chemokine biology Chemistry medicine.medical_treatment Interleukin-8 Cell Biology medicine.disease Biochemistry Article Interferon-gamma STAT1 Transcription Factor Immune system Cytokine biology.protein Cancer research medicine Humans Phosphorylation Female Interleukin 8 STAT1 Antibody Ovarian cancer |
| Zdroj: | Int J Biochem Cell Biol |
| ISSN: | 1357-2725 |
| Popis: | Interferon-γ (IFNγ) is a pleiotropic cytokine that has a crucial role in immune response and tumor immunity. Because of its anti-tumor effects, IFNγ has been used in cancer treatment. However, IFNγ also has tumor-promoting functions that are less well understood. Here, we show that IFNγ induces expression of the pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8) in ovarian cancer (OC) cells. The IFNγ-induced IL-8 expression is dependent on JAK1, STAT1, and p65 NFκB, and is associated with an increased occupancy of K314/315 acetylated p65 NFκB and Ser-727 phosphorylated STAT1 at the IL-8 promoter. Neutralization of IL-8 using anti-IL-8 antibody reduces IFNγ-induced migration of OC cells, and their invasion ability in 3D spheroids. Together, these findings identify IL-8 as a novel target induced by IFNγ/JAK1/STAT1/p65 NFκB signaling, and indicate that the IFNγ-induced IL-8 contributes to IFNγ pro-tumorigenic effects in ovarian cancer cells. |
| Databáze: | OpenAIRE |
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