Bacterial detection using bacteriophages and gold nanorods by following time-dependent changes in Raman spectral signals
| Autor: | Farzaneh Moghtader, Aysel Tomak, Hadi M. Zareie, Erhan Pişkin |
|---|---|
| Přispěvatelé: | Izmir Institute of Technology. Materials Science and Engineering |
| Rok vydání: | 2018 |
| Předmět: |
Ammonium bromide
Time Factors Biomedical Engineering time dependent Pharmaceutical Science Medicine (miscellaneous) 02 engineering and technology Biosensing Techniques Photochemistry medicine.disease_cause Spectrum Analysis Raman 01 natural sciences Raman spectral analysis Time dependent symbols.namesake chemistry.chemical_compound T4-bacteriophages medicine Escherichia coli Bacteriophage T4 Spectral analysis Plasmonic nanoparticles Nanotubes Bacterial detection biology Chemistry 010401 analytical chemistry Pathogenic bacteria General Medicine 021001 nanoscience & nanotechnology biology.organism_classification gold nanorods Gold nanorods 0104 chemical sciences symbols Nanorod Gold 0210 nano-technology Raman spectroscopy Bacteria Biotechnology |
| Popis: | PubMed: 29583029 This study attemps to develop bacterial detection strategies using bacteriophages and gold nanorods (GNRs) by Raman spectral analysis. Escherichia coli was selected as the target and its specific phage was used as the bioprobe. Target bacteria and phages were propagated/purified by traditional techniques. GNRs were synthesized by using hexadecyltrimethyl ammonium bromide (CTAB) as stabilizer. A two-step detection strategy was applied: Firstly, the target bacteria were interacted with GNRs in suspensions, and then they were dropped onto silica substrates for detection. It was possible to obtain clear surface-enchanced Raman spectroscopy (SERS) peaks of the target bacteria, even without using phages. In the second step, the phage nanoemulsions were droped onto the bacterial-GNRs complexes on those surfaces and time-dependent changes in the Raman spectra were monitored at different time intervals upto 40 min. These results demonstrated that how one can apply phages with plasmonic nanoparticles for detection of pathogenic bacteria very effectively in a quite simple test. |
| Databáze: | OpenAIRE |
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