Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Autor: Gertrude N. Uzoewulu, Nanmdi Emenyonu, Saddiq T. Abdurrahman, J. O. Lawson, Juliana N Ebisike, Mohammed A. Yassin, Christopher M. Parry, Lovett Lawson, Luis E. Cuevas, Olumide M. Sogaolu
Rok vydání: 2013
Předmět:
Male
Antitubercular Agents
Multi-drug resistant tuberculosis
Medicine
Child
Aged
80 and over
Medicine(all)
biology
Multi-drug-resistant tuberculosis
Isoniazid
General Medicine
Middle Aged
First-line anti-TB drugs
Streptomycin
wf_360
Female
medicine.symptom
Research Article
Drug sensitivity
medicine.drug
Adult
medicine.medical_specialty
Tuberculosis
Adolescent
qw_125
Nigeria
Microbial Sensitivity Tests
qv_38
General Biochemistry
Genetics and Molecular Biology
qw_45
Mycobacterium tuberculosis
Young Adult
Internal medicine
Humans
Liquid culture
Tuberculosis
Pulmonary
Ethambutol
Aged
Solid culture
Biochemistry
Genetics and Molecular Biology(all)
business.industry
bacterial infections and mycoses
biology.organism_classification
medicine.disease
Cross-Sectional Studies
Immunology
wf_220
Sputum
business
Rifampicin
Zdroj: BMC Research Notes
ISSN: 1756-0500
DOI: 10.1186/1756-0500-6-215
Popis: Background\ud This study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.\ud \ud Methods\ud This was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.\ud \ud Results\ud 527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.\ud \ud Conclusions\ud There was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.
Databáze: OpenAIRE
Externí odkaz: