Expression of integrins of the β1 family in thyroid cells from patients with Graves' disease in vivo and in vitro
| Autor: | F. Mueller, Guido Rossi, A. Casamassima, Salvatore Salzano, Luigi Marzano, Gianfranco Fenzi, Mario Vitale, Stefano De Riu |
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| Přispěvatelé: | Vitale, Mario, De Riu, S., Fenzi, G. F., Casamassima, A., Salzano, S., Müeller, F., Marzano, L. A., Rossi, G. |
| Rok vydání: | 1999 |
| Předmět: |
endocrine system
medicine.medical_specialty Graves' disease Integrin Population Cell Thyroid Gland Biology Biochemistry Follicular cell Internal medicine medicine Humans Fluorometry education Cells Cultured ICAM-1 education.field_of_study Cell adhesion molecule Integrin beta1 Thyroid General Medicine Flow Cytometry medicine.disease Molecular biology Graves Disease Endocrinology medicine.anatomical_structure biology.protein |
| Zdroj: | Biochimie. 81:477-484 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/s0300-9084(99)80098-4 |
| Popis: | The expression of the beta1 family of integrins was determined in thyroid follicular cells from patients with Graves' disease (GD). Integrin expression was quantitated by flow fluorocytometry of single cell suspensions with antibodies against the common beta1 chain and the alpha1-alpha6 subunits. Results indicated that also in thyroid glands of GD, as previously observed in nodular goiters, two follicular cell populations with different patterns of beta1 integrin expression coexist (VLAalpha3beta1 and VLAalpha1,3,5,6beta1). The VLAalpha1,3,5,6beta1 thyrocyte population in GD was more abundant than in nodular goiters, ranging from 40 to 70% of the total follicular cells and the overall expression of the beta1 integrins was a two-fold higher. In thyrocytes from patients with GD cultured in vitro, alpha3 and alpha2 expression was regulated by cell-to-cell contact as previously described in normal thyroid cells, while the expression of alpha1, alpha5 and alpha6 was quickly lost during the culture. Our data suggest that the integrin profile of the VLAalpha1,3,5,6beta1 thyrocyte population in GD is induced by micro-environmental conditions rather than being the expression of a constitutive phenotype. |
| Databáze: | OpenAIRE |
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