Diverse laboratory colonies ofAedes aegyptiharbor the same adult midgut bacterial microbiome

Autor: Anubis Vega-Rúa, Martin N. Mayanja, Isabelle Dusfour, Veasna Duong, Alain Kohl, Christophe Paupy, Christiane Bouchier, Amine Ghozlane, Laura B. Dickson, Stevenn Volant, Louis Lambrechts, Julius J. Lutwama, Laurence Ma, Davy Jiolle
Přispěvatelé: Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Centre de Recherche et Innovation Technologique (CITECH), Institut Pasteur [Paris] (IP), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de la Guyane, Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre International de Recherches Médicales de Franceville (CIRMF), Uganda Virus Research Institute, Entebbe, Uganda, MRC - University of Glasgow Centre for Virus Research, Institut Pasteur du Cambodge, This work was supported by Agence Nationale de la Recherche (grants ANR-16-CE35-0004-01 and ANR-17-ERC2-0016-01 to LL), the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID to LL), the City of Paris Emergence(s) program in Biomedical Research (to LL), the European Union’s Horizon 2020 research and innovation programme under ZikaPLAN grant agreement No 734584 (to LL), the European Union and Guadeloupe Region (Programme Opérationnel FEDER-Guadeloupe-Conseil Régional 2014-2020, grant 2015-FED-192 to AVR) and the United Kingdom Medical Research Council (grant MC_UU_12014 to AK). The Genomics Facility is a member of the 'France Génomique' consortium (grant ANR10-INBS-09-08 to CB). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication., We are grateful to Gordana Rašić and Ary Hoffmann for providing the mosquito colony from Cairns and to Borin Peng for the mosquito collection in Phnom Penh. We thank Claire Valiente Moro, Guillaume Minard and the Lambrechts lab members for their insights., ANR-16-CE35-0004,MOSQUIBIOTA,Contribution de la diversité bactérienne intestinale à la capacité vectorielle d'Aedes aegypti(2016), ANR-17-ERC2-0016,GxG,Base génétique de la spécificité génotype-génotype dans l'interaction naturelle entre un virus et son insecte vecteur(2017), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 734584,ZikaPLAN, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]
Rok vydání: 2017
Předmět:
Zdroj: Parasites & Vectors
Parasites & Vectors, 2018, 11 (1), pp.207. ⟨10.1186/s13071-018-2780-1⟩
Parasites and Vectors
Parasites and Vectors, BioMed Central, 2018, 11 (1), pp.207. ⟨10.1186/s13071-018-2780-1⟩
Parasites & Vectors, Vol 11, Iss 1, Pp 1-8 (2018)
ISSN: 1756-3305
DOI: 10.1101/200659
Popis: BackgroundHost-associated microbes, collectively known as the microbiota, play an important role in the biology of multicellular organisms. In mosquito vectors of human pathogens, the gut bacterial microbiota influences vectorial capacity and has become the subject of intense study. In laboratory studies of vector biology, genetic effects are often inferred from differences between geographically and genetically diverse colonies of mosquitoes that are reared in the same insectary. It is unclear, however, to what extent genetic effects can be confounded by uncontrolled differences in the microbiota composition among mosquito colonies. To address this question, we used 16S metagenomics to compare the midgut bacterial microbiome of six recent laboratory colonies ofAedes aegyptirepresenting the geographical range and genetic diversity of the species.ResultsWe found that the diversity, abundance, and community structure of the midgut bacterial microbiome was remarkably similar among the six different colonies ofAe. aegypti, regardless of their geographic origin. We also confirmed the relatively low complexity of bacterial communities inhabiting the mosquito midgut.ConclusionsOur finding that geographically diverse colonies ofAe. aegyptireared in the same insectary harbor a similar gut bacterial microbiome supports the conclusion that the gut microbiota of adult mosquitoes is environmentally determined regardless of the host genotype. Thus, uncontrolled differences in microbiota composition are unlikely to represent a significant confounding factor in genetic studies of vector biology.
Databáze: OpenAIRE
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