Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes
| Autor: | Kazuyoshi Ikuta, Jun Komano, Piraporn Utachee, Panadda Dhepakson, Panasda Isarangkura-na-ayuthaya, Hironori Sato, Masanori Kameoka, Kenzo Tokunaga, Piyamat Jinnopat, Yukiko Kitagawa, Naoki Yamamoto, Shinobu Oguchi, Yukikazu Natori |
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| Rok vydání: | 2007 |
| Předmět: |
Small interfering RNA
Biophysics Clone (cell biology) Cell Biology Transfection Biology Virus Replication Biochemistry Virology Cell Line Gene Expression Regulation Viral replication RNA interference Cell culture HIV-1 Suppressor Factors Immunologic Humans RNA Small Interfering Molecular Biology Gene Gene Library Host factor |
| Zdroj: | Biochemical and Biophysical Research Communications. 359:729-734 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.05.173 |
| Popis: | We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication. |
| Databáze: | OpenAIRE |
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