Dye-impregnated paper strips for staining bacteria
| Autor: | Harry R. Elston, Herbert J. Quigley |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Paper
Bacteriological Techniques biology Bacteria Staining and Labeling business.industry Sputum Nocardia General Medicine bacterial infections and mycoses biology.organism_classification Stain Microbiology Staining Mycobacterium Evaluation Studies as Topic Methods Wound Infection Medicine Humans business Coloring Agents |
| Zdroj: | American journal of clinical pathology. 60(4) |
| ISSN: | 0002-9173 |
| Popis: | Paper strips impregnated with stains and chemicals for bacterial staining (Gugol Scientific Corporation, distributed by Wampole Laboratories) were evaluated. The quality of staining 100 Gram-positive and 80 Gram-negative microorganisms was compared with Hucker's modification. Over-decolorization of strip-stained Gram-positive organisms was the most frequent cause of inconsistency. Paper strip “acid-fast” stain was compared with the Ziehl-Neelsen technic for 90 mycobacteria and nine Nocardia strains. Mycobacteria and Nocardia were consistently well stained by the paper strip technic; however, Nocardia were not stained by the true acid-fast method. Ninety-five clinical smears, stripstained by physicians on the wards, were evaluated. Ease of performance and stability of reagents make the paper strip technics practical for use in clinics, wards, and small bncteriologic laboratories. |
| Databáze: | OpenAIRE |
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