Measurement of pre- and post-synaptic proteins in cerebral cortex: effects of post-mortem delay
| Autor: | Shelley J Allen, Lai Khai Siew, David Dawbarn, Gordon K. Wilcock, Seth Love |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
medicine.medical_specialty Time Factors Synaptophysin Nerve Tissue Proteins Internal medicine Cortex (anatomy) medicine Syntaxin Animals Humans Incubation Aged Temporal cortex Aged 80 and over Cerebral Cortex biology Qa-SNARE Proteins General Neuroscience Intracellular Signaling Peptides and Proteins Membrane Proteins Human brain Middle Aged Rats Endocrinology medicine.anatomical_structure nervous system Cerebral cortex Disks Large Homolog 4 Protein Postmortem Changes Synapses biology.protein Neuroscience |
| Popis: | Assessments of synaptic density in human brain are often based on measurements of synaptic proteins. Little information is available on their post-mortem stability. We have investigated this by ELISAs of the pre-synaptic proteins syntaxin and synaptophysin, and the post-synaptic protein PSD-95, in rat and human cortex. The rat brains were cooled in situ from 37 to 20 or 4 degrees C over 3 h, and then kept at 20 or 4 degrees C for a further 24-72 h, to simulate post-mortem storage at room temperature or in a mortuary refrigerator. Synaptophysin and PSD-95 levels in rat cerebral cortex were not significantly decreased after 72 h of incubation at 20 degrees C. Syntaxin was stable for 24 h but decreased by 39-44% at 48-72 h. Storage at 4 degrees C resulted in a similar reduction of syntaxin levels over 72 h. In human brain tissue from 160 people aged 24-102 years, post-mortem delay had little effect on synaptic protein levels in superior temporal cortex, but was associated with a decline in PSD-95 and syntaxin in mid-frontal cortex after 24 h. The more robust stability of synaptophysin may be related to its multi-transmembrane structure. |
| Databáze: | OpenAIRE |
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