Identification and stable expression of vitellogenin receptor through vitellogenesis in the European eel
| Autor: | Marina Morini, Sylvie Dufour, Gersende Maugars, S. Baloche, Juan F. Asturiano, Anne-Gaëlle Lafont, Luz Pérez |
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| Přispěvatelé: | Centre de Ressources Biologiques APHP-SU (PASS-CRB-APHP-SU), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MESH: Vitellogenins
Teleost [SDV]Life Sciences [q-bio] MESH: Vitellogenesis PRODUCCION ANIMAL MESH: Sexual Maturation Oogenesis Vitellogenins 0302 clinical medicine Transcription (biology) MESH: Pituitary Gland MESH: Animals Sexual Maturation Receptor MESH: Receptors Cell Surface 0303 health sciences teleost biology Anguilla anguilla oogenesis Reproduction Vitellogenesis MESH: Gene Expression Regulation Cell biology Animal culture medicine.anatomical_structure Liver Pituitary Gland Female MESH: Ovary endocrine system MESH: Egg Proteins food.ingredient animal structures Receptors Cell Surface SF1-1100 MESH: Oocytes reproduction 03 medical and health sciences Vitellogenin food Yolk medicine Animals MESH: Anguilla lipoprotein receptor 8 030304 developmental biology Lipoprotein receptor 8 Egg Proteins Ovary Oocyte Anguilla Gene Expression Regulation Receptors LDL MESH: Receptors LDL LDL receptor biology.protein Oocytes Animal Science and Zoology MESH: Female 030217 neurology & neurosurgery MESH: Liver |
| Zdroj: | animal animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2020, 14 (6), pp.1213-1222. ⟨10.1017/S1751731119003355⟩ RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Animal, Vol 14, Iss 6, Pp 1213-1222 (2020) Animal Animal, Published by Elsevier (since 2021) / Cambridge University Press (until 2020), 2020, 14 (6), pp.1213-1222. ⟨10.1017/S1751731119003355⟩ |
| ISSN: | 1751-7311 1751-732X |
| DOI: | 10.1017/S1751731119003355⟩ |
| Popis: | [EN] In teleosts, vitellogenin (Vtg) is a phospholipoglycoprotein synthesized by the liver, released into the blood circulation and incorporated into the oocytes via endocytosis mediated by the Vtg receptor (VTGR) to form the yolk granules. The VTGR is crucial for oocyte growth in egg-laying animals but is also present in non-oviparous vertebrates, such as human. The VTGR belongs to the low-density lipoprotein receptor superfamily (LDLR) and is also named very-low-density lipoprotein receptor (VLDLR). In this study, we identified and phylogenetically positioned the VTGR of a basal teleost, the European eel, Anguilla anguilla. We developed quantitative real-time PCR (qRT-PCR) and investigated the tissue distribution of vtgr transcripts. We compared by qRT-PCR the ovarian expression levels of vtgr in juvenile yellow eels and pre-pubertal silver eels. We also analyzed the regulation of ovarian vtgr expression throughout vitellogenesis in experimentally matured eels. The Vtg plasma level was measured by homologous ELISA experimental maturation. Our in silico search and phylogenetical analysis revealed a single vtgr in the European eel, orthologous to other vertebrate vtgr. The qRT-PCR studies revealed that vtgr is mainly expressed in the ovary and also detected in various other tissues such as brain, pituitary, gill, fat, heart, and testis, suggesting some extra-ovarian functions of VTGR. We showed that vtgr is expressed in ovaries of juvenile yellow eels with no higher expression in pre-pubertal silver eels nor in experimentally matured eels. This suggests that vtgr transcription already occurs during early pre-vitellogenesis of immature eels and is not further activated in vitellogenic oocytes. European eel Vtg plasma level increased throughout experimental maturation in agreement with previous studies. Taken together, these results suggest that vtgr transcript levels may not be a limiting step for the uptake of Vtg by the oocyte in the European eel. M.M., A.G.L. and S.D. were granted with Short Term Scientific Missions by the COST Office (COST Action FA1205: AQUAGAMETE). S.D. was also awarded with a grant from the UPV's School of Doctorate (Accion para la Internacionalizacion de los Programas de Doctorado) in 2017. We thank E. Feunteun and colleagues, MNHN, Dinard, for the ovarian samples from eels of the Fremur, France. |
| Databáze: | OpenAIRE |
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