Polyoxygenated Dysidea Sterols That Inhibit the Binding of [I125] IL-8 to the Human Recombinant IL-8 Receptor Type A
| Autor: | Ronald J. Quinn, John N. A. Hooper, Priscila de Almeida Leone, Joanne Redburn |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Magnetic Resonance Spectroscopy
Spectrophotometry Infrared Stereochemistry Pharmaceutical Science Fractionation Receptor type Mass Spectrometry Receptors Interleukin-8A Analytical Chemistry law.invention Iodine Radioisotopes Antigens CD law Drug Discovery Animals Humans Interleukin 8 Pharmacology biology Il 8 receptor Organic Chemistry Australia Receptors Interleukin biology.organism_classification Recombinant Proteins Porifera Sponge Complementary and alternative medicine Northern australia Recombinant DNA Molecular Medicine Spectrophotometry Ultraviolet Two-dimensional nuclear magnetic resonance spectroscopy Protein Binding |
| Zdroj: | Journal of Natural Products. 63:694-697 |
| ISSN: | 1520-6025 0163-3864 |
| DOI: | 10.1021/np9904657 |
| Popis: | The combined CH(2)Cl(2) and MeOH crude extract of a new species of the marine sponge Dysidea, collected in Northern Australia was found to inhibit the binding of [I125] interleukin-8 [IL-8] to the human recombinant IL-8 receptor type A at 500 microg/mL. Bioassay-guided fractionation led to the isolation of three new polyoxygenated sterols 3, 4, and 5. Their structures were assigned on the basis of 1D and 2D NMR experiments, and relative stereochemistries were established by ROESY correlations and analysis of coupling constants. The IC(50) values for inhibition of IL-8Ra for sterols 3, 4, and 5 were 20, 5.5, and 4.5 microM, respectively. |
| Databáze: | OpenAIRE |
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