Effect of individual Fc methionine oxidation on FcRn binding: Met252 oxidation impairs FcRn binding more profoundly than Met428 oxidation
| Autor: | Robert F. Kelley, Taylor Zhang, Xuan Gao, Junyan A. Ji, Mary E.M. Cromwell, Jun Liu, Kai Zheng, William Mcgreevy, Y. John Wang, Michael W. Laird, Karthik Veeravalli |
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| Rok vydání: | 2014 |
| Předmět: |
Mutation
Methionine Binding Sites biology Chemistry Mutant Mutagenesis Histocompatibility Antigens Class I Pharmaceutical Science Plasma protein binding Receptors Fc Surface Plasmon Resonance medicine.disease_cause Fragment crystallizable region Immunoglobulin Fc Fragments chemistry.chemical_compound Neonatal Fc receptor Biochemistry Immunoglobulin G medicine biology.protein Humans Antibody Oxidation-Reduction |
| Zdroj: | Journal of pharmaceutical sciences. 104(2) |
| ISSN: | 1520-6017 |
| Popis: | The long serum half-lives of mAbs are conferred by pH-dependent binding of IgG-Fc to the neonatal Fc receptor (FcRn). The Fc region of human IgG1 has three conserved methionine residues, Met252, Met358, and Met428. Recent studies showed oxidation of these Met residues impairs FcRn binding and consequently affects pharmacokinetics of therapeutic antibodies. However, the quantitative effect of individual Met oxidation on Fc-FcRn binding has not been addressed. This information is valuable for defining critical quality attributes. In the present study, two sets of homodimeric site-directed IgG1 mutations were generated to understand how individual Fc Met oxidation affects FcRn binding. The first approach used Met to Leu mutants to block site-specific Met oxidation. In the other approach, Met to Gln mutants were designed to mimic site-specific Met oxidation. Both mutagenesis approaches show that either Met252 or Met428 oxidation alone significantly impairs Fc-FcRn binding. Met252 oxidation has a more deleterious effect on FcRn binding than M428 oxidation, whereas Met428 oxidation has a bigger destabilization effect on the thermal stability. Our results also show that Met358 oxidation does not affect FcRn binding. In addition, our study suggests that Met to Gln mutation may serve as an important tool to understand Met oxidation. |
| Databáze: | OpenAIRE |
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