Experimental Infection of Pigs with the Human 1918 Pandemic Influenza Virus
| Autor: | Shawn Babiuk, Jürgen A. Richt, Porntippa Lekcharoensuk, James Neufeld, Hana M. Weingartl, Kelly M. Lager, Randy A. Albrecht, Carissa Embury-Hyatt, Terrence M. Tumpey, Peter Marszal, Adolfo García-Sastre |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Swine
animal diseases viruses Immunology Population Orthomyxoviridae Virulence Biology medicine.disease_cause Microbiology Antibodies Virus Disease Outbreaks Mice Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Virology Pandemic medicine Influenza A virus Animals education education.field_of_study Influenza A Virus H3N2 Subtype virus diseases respiratory system History 20th Century biology.organism_classification Survival Rate Disease Models Animal Titer medicine.anatomical_structure Insect Science RNA Viral Pathogenesis and Immunity Female Respiratory tract |
| Zdroj: | Journal of Virology. 83:4287-4296 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.02399-08 |
| Popis: | Swine influenza was first recognized as a disease entity during the 1918 “Spanish flu” pandemic. The aim of this work was to determine the virulence of a plasmid-derived human 1918 pandemic H1N1 influenza virus (reconstructed 1918, or 1918/rec, virus) in swine using a plasmid-derived A/swine/Iowa/15/1930 H1N1 virus (1930/rec virus), representing the first isolated influenza virus, as a reference. Four-week-old piglets were inoculated intratracheally with either the 1930/rec or the 1918/rec virus or intranasally with the 1918/rec virus. A transient increase in temperature and mild respiratory signs developed postinoculation in all virus-inoculated groups. In contrast to other mammalian hosts (mice, ferrets, and macaques) where infection with the 1918/rec virus was lethal, the pigs did not develop severe respiratory distress or become moribund. Virus titers in the lower respiratory tract as well as macro- and microscopic lesions at 3 and 5 days postinfection (dpi) were comparable between the 1930/rec and 1918/rec virus-inoculated animals. In contrast to the 1930/rec virus-infected animals, at 7 dpi prominent lung lesions were present in only the 1918/rec virus-infected animals, and all the piglets developed antibodies at 7 dpi. Presented data support the hypothesis that the 1918 pandemic influenza virus was able to infect and replicate in swine, causing a respiratory disease, and that the virus was likely introduced into the pig population during the 1918 pandemic, resulting in the current lineage of the classical H1N1 swine influenza viruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|