MTERF3, the most conserved member of the mTERF-family, is a modular factor involved in mitochondrial protein synthesis
| Autor: | Caterina Manzari, Maria Nicola Gadaleta, Paola Loguercio Polosa, Palmiro Cantatore, Marina Roberti, Francesco Bruni |
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| Rok vydání: | 2006 |
| Předmět: |
Sequence analysis
Mitochondrial translation RNA Mitochondrial Molecular Sequence Data Biophysics Mitochondrion Biology Biochemistry Mitochondrial Proteins Transcription (biology) mitochondrial protein synthesis Animals Drosophila Proteins Amino Acid Sequence RNA Messenger Conserved Sequence HSPA9 Genetics Cell Biology Mitochondrial carrier mitochondria Drosophila melanogaster Gene Expression Regulation Protein Biosynthesis MTERF3 DNAJA3 RNA Drosophila RNA Interference ATP–ADP translocase mTERF Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1757(9-10):1199-1206 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2006.04.026 |
| Popis: | The MTERF-family is a wide family of proteins identified in Metazoa and plants which includes the known mitochondrial transcription termination factors. With the aim to shed light on the function of MTERF-family members in Drosophila, we performed the cloning and characterization of D-MTERF3, a component of the most conserved group of this family. D-MTERF3 is a mitochondrial protein of 323 amino acids. Sequence analysis in seven different organisms showed that the protein contains five conserved “mTERF-motifs”, three of which include a leucine zipper-like domain. D-MTERF3 knock-down, obtained by RNAi in D.Mel-2 cells, did not affect mitochondrial replication and transcription. On the contrary, it decreased to a variable extent the rate of labelling of about half of the mitochondrial polypeptides, with ND1 being the most affected by D-MTERF3 depletion. These results indicate that D-MTERF3 is involved in mitochondrial translation. This role, likely based on protein–protein interactions, may be exerted either through a direct interaction with the translation machinery or by bridging the mitochondrial transcription and translation apparatus. |
| Databáze: | OpenAIRE |
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