Effects of mixed meal tolerance test on gastric emptying, glucose and lipid homeostasis in obese nonhuman primates
| Autor: | Xiaoli Wang, Kamal Albarazanji, Bin Gao, Yong-Fu Xiao, Yixin Jim Wang, Andrea R Nawrocki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty medicine.medical_treatment Science Incretin 030209 endocrinology & metabolism Gastric Inhibitory Polypeptide Incretins Article Gastrointestinal Hormones 03 medical and health sciences 0302 clinical medicine Insulin-Secreting Cells Internal medicine medicine Animals Homeostasis Insulin Glucose homeostasis Acetaminophen Meal Multidisciplinary C-Peptide Gastric emptying business.industry digestive oral and skin physiology Reproducibility of Results Type 2 Diabetes Mellitus Glucose Tolerance Test Animal Feed Lipids Metabolic syndrome Macaca fascicularis Glucose Metabolism 030104 developmental biology Endocrinology Diabetes Mellitus Type 2 Gastric Emptying Medicine business Hormone medicine.drug |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Meal ingestion elicits a variety of neuronal, physiological and hormonal responses that differ in healthy, obese or diabetic individuals. The mixed meal tolerance test (MMTT) is a well-established method to evaluate pancreatic β-cell reserve and glucose homeostasis in both preclinical and clinical research in response to calorically defined meal. Nonhuman primates (NHPs) are highly valuable for diabetic research as they can naturally develop type 2 diabetes mellitus (T2DM) in a way similar to the onset and progression of human T2DM. The purpose of this study was to investigate the reproducibility and effects of a MMTT containing acetaminophen on plasma glucose, insulin, C-peptide, incretin hormones, lipids, acetaminophen appearance (a surrogate marker for gastric emptying) in 16 conscious obese cynomolgus monkeys (Macaca fascicularis). Plasma insulin, C-peptide, TG, aGLP-1, tGIP, PYY and acetaminophen significantly increased after meal/acetaminophen administration. A subsequent study in 6 animals showed that the changes of plasma glucose, insulin, C-peptide, lipids and acetaminophen were reproducible. There were no significant differences in responses to the MMTT among the obese NHPs with (n = 11) or without (n = 5) hyperglycemia. Our results demonstrate that mixed meal administration induces significant secretion of several incretins which are critical for maintaining glucose homeostasis. In addition, the responses to the MMTTs are reproducible in NHPs, which is important when the MMTT is used for evaluating post-meal glucose homeostasis in research. |
| Databáze: | OpenAIRE |
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