A microfluidic platform for high‐purity cell free DNA extraction from plasma for non‐invasive prenatal testing

Autor: Lindsay Schneider, Thomas Usherwood, Anubhav Tripathi
Rok vydání: 2022
Předmět:
Zdroj: Prenatal Diagnosis. 42:240-253
ISSN: 1097-0223
0197-3851
DOI: 10.1002/pd.6092
Popis: Increase the yield and purity of cell-free DNA (cfDNA) extracted from plasma for non-invasive prenatal testing (NIPT) as inefficiencies in this extraction and purification can dramatically affect the sensitivity and specificity of the test.This work integrates cfDNA extraction from plasma with a microfluidic chip platform by combining magnetic bead-based extraction and electroosmotic flow on the microfluidic chip. Various wash buffers and voltage conditions were simulated using COMSOL Multiphysics Modeling and tested experimentally.When performing the first wash step of this assay on the microfluidic chip with 300 V applied across the channel there was a six-fold increase in the AThis technique has the potential to improve NIPT outcomes and other clinically relevant workflows that use cfDNA as an analyte such as cancer detection.
Databáze: OpenAIRE
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