Experimental Evaluation of a Cell Module for Hybrid Liver Support
| Autor: | Dimitrios Kardassis, Patzold A, Kosan M, M. Schön, Tietze S, Gero Puhl, Belal R, Peter Neuhaus, Kosan B, Sültmann J, Böhmer C, Igor M. Sauer, Ingenlath M, M. Kraemer, Janke J, J.K. Unger, Jörg C. Gerlach, Christian W. Müller, Rolf Rossaint, B Busse, Botsch M, P. Lemmens, E Mönch |
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| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Pathology Swine medicine.medical_treatment 030232 urology & nephrology Biomedical Engineering Urology Medicine (miscellaneous) Bioengineering In Vitro Techniques 030204 cardiovascular system & hematology Biology Biomaterials 03 medical and health sciences 0302 clinical medicine Parenchyma medicine Animals Hepatectomy Bile duct General Medicine Liver Failure Acute Total Hepatectomy Liver Artificial Survival Analysis medicine.anatomical_structure Hepatocyte Models Animal Vascular resistance Female Ligation Artery |
| Zdroj: | The International Journal of Artificial Organs. 24:793-798 |
| ISSN: | 1724-6040 0391-3988 |
| DOI: | 10.1177/039139880102401105 |
| Popis: | Aim of the study was to evaluate a hybrid liver support system in a porcine model of acute liver failure, after hepatectomy. Pigs with a body weight of 70±18 kg underwent total hepatectomy and porto - cavo - caval shunting as well as ligation of the bile duct and the hepatic artery. Control animals were connected to the system (including capillary membrane plasma separation) containing a four compartment bioreactor with integral oxygenation and decentralized mass exchange but without liver cells. The treatment group received hybrid liver support with the same system including 370±42 g primary isolated porcine parenchymal liver cells in co-culture with hepatocyte nursing cells, tissue engineered to liver- like structures at high density. Treatment started after complete recovery from anesthesia and was performed continuously. A positive influence on peripheral vascular resistance and a reduced need of catecholamine dosage was observed in the treatment group. Hybrid liver support with a cell module upscaled for clinical application significantly prolonged survival time in animals after hepatectomy with the longest survival being 26 hours in the control group an 57 hours in the treatment group. |
| Databáze: | OpenAIRE |
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