Physicochemical and immunological characterization of chitosan-coated bacteriophage nanoparticles for in vivo mycotoxin modeling
| Autor: | Isabel B. Yamanaka, Rilton Alves de Freitas, Carla Yoko Tanikawa de Andrade, Sabrina Karim Silva, Juliana de Moura, Guilherme Fadel Picheth, Laís S. Schlichta, Larissa Magalhães Alvarenga, Luiz Felipe Caron |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aflatoxin B1 Phage display Immunogen Polymers and Plastics Epitope Bacteriophage Mice 03 medical and health sciences Antigen Peptide Library Materials Chemistry Animals Bacteriophages Peptide library Chitosan Vaccines biology Mimotope Chemistry Immunogenicity Organic Chemistry technology industry and agriculture food and beverages biology.organism_classification 030104 developmental biology Biochemistry Nanoparticles Female |
| Zdroj: | Carbohydrate Polymers. 185:63-72 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2017.12.063 |
| Popis: | To propose a novel modeling of aflatoxin immunization and surrogate toxin conjugate from AFB1 vaccines, an immunogen based on the mimotope, (i.e. a peptide-displayed phage that mimics aflatoxins epitope without toxin hazards) was designed. The recombinant phage 3P30 was identified by phage display technology and exhibited the ability to bind, dose dependent, specifically to its cognate target - anti-AFB1 antibody. In immunization assay, the phage-displayed mimotope and its peptide chemically synthesized were able to induce specific anti-AFB1 antibodies, indicating the proof of concept for aflatoxin mimicry. Furthermore, the phage 3P30 was homogeneously coated with chitosan, which also provided a tridimensional matrix network for mucosal delivery. After intranasal immunization, chitosan coated phages improved specific immunogenicity compared to the free antigen. It can be concluded that affinity-selected phage may contribute to the rational design of epitope-based vaccines in a prospectus for the control of aflatoxins and possibly other mycotoxins, and that chitosan coating improved the vectorization of the vaccine by the mucosal route. |
| Databáze: | OpenAIRE |
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