Distribution patterns of three sodium channel mutations associated with pyrethroid resistance in Rhipicephalus (Boophilus) microplus populations from North and South America, South Africa and Australia
| Autor: | Deanna Bodine, Bruno Betschart, Heinz Sager, Felix D. Guerrero, Léonore Lovis, Robert J. Miller |
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| Rok vydání: | 2012 |
| Předmět: |
Synthetic pyrethroid resistance
Pharmacology Genetics biology Rhipicephalus (Boophilus) microplus Ecology Wild type Multiplex PCR Tick Flumethrin biology.organism_classification Article Cypermethrin Rhipicephalus chemistry.chemical_compound Infectious Diseases chemistry Mutation parasitic diseases Mutation (genetic algorithm) Genotype Pharmacology (medical) Parasitology Allele frequency |
| Zdroj: | International Journal for Parasitology: Drugs and Drug Resistance. 2:216-224 |
| ISSN: | 2211-3207 |
| Popis: | Resistance to synthetic pyrethroids (SP) in the cattle tick Rhipicephalus (Boophilus) microplus is widespread throughout its distribution area. Three single nucleotide substitutions identified in Domains II and III of the sodium channel gene of R. (B.) microplus are known to be associated with target site pyrethroid resistance. We developed a multiplex PCR using allele-specific primers to amplify wild type or mutated genotypes of the three mutations simultaneously. This assay was used to screen tick samples originating from Brazil, Argentina, Mexico, South Africa and Australia whose phenotype to flumethrin and cypermethrin had been determined by the use of the Larval Tarsal test (LTT) or the Larval Packet Test (LPT). These mutations were found to have distinct geographical distributions and result in different resistance phenotypes. The L64I Domain II mutation conferring resistance to several SP compounds was found in all the Brazilian, Argentinean and Australian populations and in one South African population, with frequencies between 38% and 100% in flumethrin and cypermethrin resistant populations. In contrast, this mutation was not found in samples from Mexico, while the Domain III mutation was found exclusively in this country. The G72V Domain II flumethrin-specific mutation was found in a single Australian population, with a very low resistant allele frequency (3%). The homozygous resistant RR genotype of the L64I Domain II mutation correlated significantly with the survival rates at the discriminating doses of flumethrin and cypermethrin. This survey shows the widespread distribution of the L64I Domain II mutation and provides evidence of its geographic separation from the Domain III mutation. |
| Databáze: | OpenAIRE |
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