The Early Isoform of Disabled-1 Functions Independently of Reelin-Mediated Tyrosine Phosphorylation in Chick Retina
| Autor: | Roseline Godbout, Cairine Logan, Darryl D. Glubrecht, Zhihua Gao, Elizabeth A. Monckton |
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| Rok vydání: | 2010 |
| Předmět: |
Retinal Ganglion Cells
Cell Adhesion Molecules Neuronal Cellular differentiation Mitosis Nerve Tissue Proteins Chick Embryo Cell fate determination Retina chemistry.chemical_compound medicine Animals Protein Isoforms Reelin Phosphorylation Tyrosine Molecular Biology Cells Cultured Adaptor Proteins Signal Transducing Cell Proliferation Neurons Extracellular Matrix Proteins biology Stem Cells Serine Endopeptidases Gene Expression Regulation Developmental Nuclear Proteins Cell Differentiation Tyrosine phosphorylation Articles Cell Biology DAB1 Cell biology Reelin Protein medicine.anatomical_structure nervous system chemistry Gene Knockdown Techniques biology.protein Amyloid Precursor Protein Secretases Chickens |
| Zdroj: | Molecular and Cellular Biology. 30:4339-4353 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.00545-10 |
| Popis: | The Reelin-Disabled-1 (Dab1) signaling pathway plays a key role in the positioning of neurons during brain development. Two alternatively spliced Dab1 isoforms have been identified in chick retina and brain: Dab1-E, expressed at early stages of development, and Dab1-L (commonly referred to as Dab1), expressed at later developmental stages. The well-studied Dab1-L serves as an adaptor protein linking Reelin signal to its downstream effectors; however, nothing is known regarding the role of Dab1-E. Here we show that Dab1-E is primarily expressed in proliferating retinal progenitor cells whereas Dab1-L is found exclusively in differentiated neuronal cells. In contrast to Dab1-L, which is tyrosine phosphorylated upon Reelin stimulation, Dab1-E is not tyrosine phosphorylated and may function independently of Reelin. Knockdown of Dab1-E in chick retina results in a significant reduction in the number of proliferating cells and promotes ganglion cell differentiation. Our results demonstrate a role for Dab1-E in the maintenance of the retinal progenitor pool and determination of cell fate. |
| Databáze: | OpenAIRE |
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