An age-dependent reversal in the protective capacities of JNK signaling shortens Caenorhabditis elegans lifespan
| Autor: | Michael Y. Shapira, Sudarshan Bhat, Timothy Wang, Ali Salehpour, Kuang-Hui Lee, Man-Wah Tan, Kwame Twumasi-Boateng, Linda Tsai |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Aging
MAP Kinase Signaling System Longevity Drug Resistance Context (language use) medicine.disease_cause antagonistic pleiotropy Animals Genetically Modified 03 medical and health sciences stress KGB-1 0302 clinical medicine RNA interference Stress Physiological medicine Animals Protein kinase A Caenorhabditis elegans Caenorhabditis elegans Proteins Transcription factor Genes Helminth 030304 developmental biology 0303 health sciences Mutation biology Kinase JNK Mitogen-Activated Protein Kinases Forkhead Transcription Factors Cell Biology Original Articles biology.organism_classification Phenotype Cell biology Gene Knockdown Techniques C. elegans Dual-Specificity Phosphatases RNA Interference JNK 030217 neurology & neurosurgery lifespan Cadmium Transcription Factors |
| Zdroj: | Aging Cell |
| ISSN: | 1474-9726 |
| Popis: | Stress-activated protein kinase (SAPK) pathways are evolutionarily conserved signaling modules that orchestrate protective responses to adverse environmental conditions. However, under certain conditions, their activation can be deleterious. Thus, activation of the c-Jun N-terminal kinase (JNK) SAPK pathway exacerbates a diverse set of pathologies, many of which are typical of old age. The contexts determining whether the outcome of JNK signaling is protective or detrimental are not fully understood. Here, we show that the age of an animal defines such a context. The Caenorhabditis elegans JNK homolog, KGB-1, provides protection from heavy metals and protein folding stress in developing animals. However, we found that with the onset of adulthood, KGB-1 activity becomes detrimental, reducing stress resistance and lifespan. Genetic analyses coupled with fluorescent imaging linked this phenotypic switch to age-dependent antagonistic modulation of DAF-16/FOXO: KGB-1 activation enhanced DAF-16 nuclear localization and transcriptional activity during development but decreased it in adults. Epistasis analyses showed that DAF-16 was necessary and sufficient to explain some of the kgb-1-dependent detrimental phenotypes, but not all. The identification of early adulthood as a point following which the contribution of KGB-1 activity reverses from beneficial to detrimental sheds new light on the involvement of JNK signaling in age-related pathologies. Furthermore, the age-dependent reversal has intriguing implications for our understanding of aging. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|