Transcriptional analysis of islets of Langerhans from organ donors of different ages

Autor: Oskar Skog, Anton Stenwall, Petr Volkov, Jonathan L.S. Esguerra, Olle Korsgren, Louise Granlund, Marcus Lundberg, Peter Seiron, Anders Hedin, Erik Renström
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Aging
Physiology
Gene Expression
Biochemistry
Transcriptome
Endocrinology
Medical Conditions
Gene expression
Insulin Secretion
Medicine and Health Sciences
Insulin
Osteopontin
Cell Cycle and Cell Division
Child
Cellular Senescence
Laser capture microdissection
Aged
80 and over
Multidisciplinary
geography.geographical_feature_category
Organic Compounds
Cell Cycle
Monosaccharides
Genomics
Cell cycle
Middle Aged
Islet
Type 2 Diabetes
Chemistry
Cell Processes
Child
Preschool
Physical Sciences
Medicine
Female
Transcriptome Analysis
Research Article
Senescence
Adult
endocrine system
Adolescent
Endocrine Disorders
Science
Carbohydrates
Biology
Andrology
Islets of Langerhans
Young Adult
Downregulation and upregulation
Genetics
Diabetes Mellitus
Humans
Aged
Diabetic Endocrinology
geography
Endocrine Physiology
Gene Expression Profiling
Organic Chemistry
Chemical Compounds
Infant
Biology and Life Sciences
Computational Biology
Cell Biology
Genome Analysis
Hormones
Glucose
Metabolic Disorders
biology.protein
Physiological Processes
Organism Development
Developmental Biology
Zdroj: PLoS ONE, Vol 16, Iss 3, p e0247888 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Insulin secretion is impaired with increasing age. In this study, we aimed to determine whether aging induces specific transcriptional changes in human islets. Laser capture microdissection was used to extract pancreatic islet tissue from 37 deceased organ donors aged 1–81 years. The transcriptomes of the extracted islets were analysed using Ion AmpliSeq sequencing. 346 genes that co-vary significantly with age were found. There was an increased transcription of genes linked to senescence, and several aspects of the cell cycle machinery were downregulated with increasing age. We detected numerous genes not linked to aging in previous studies likely because earlier studies analysed islet cells isolated by enzymatic digestion which might affect the islet transcriptome. Among the novel genes demonstrated to correlate with age, we found an upregulation of SPP1 encoding osteopontin. In beta cells, osteopontin has been seen to be protective against both cytotoxicity and hyperglycaemia. In summary, we present a transcriptional profile of aging in human islets and identify genes that could affect disease course in diabetes.
Databáze: OpenAIRE
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