Assessment of cotinine in urine and saliva of smokers, passive smokers, and nonsmokers: Method validation using liquid chromatography and mass spectrometry

Autor:	Shravanthi Daphne Anand, Niharika Sane, Vivek Benegal, P Marimutthu, Priyamvada Sharma
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Saliva Fagerstrom Test for Nicotine Dependence Chromatography business.industry passive smokers Urine Tobacco smoke 030227 psychiatry Nicotine 03 medical and health sciences Psychiatry and Mental health chemistry.chemical_compound 0302 clinical medicine chemistry Tobacco users Medicine Smoking status Original Article liquid chromatography and mass spectrometry 030212 general & internal medicine Cotinine business Secondhand smoke medicine.drug
Zdroj:	Indian Journal of Psychiatry
ISSN:	1998-3794 0019-5545
Popis:	Background: Self-report does not provide complete information about tobacco smoke exposure among users and is not relevant for secondhand exposure detection. Biochemical screening for primary metabolite of nicotine would be useful to validate the smoking status and exposure to secondhand smoke. Aims and Objectives: This study was designed to evaluate the performance of a sensitive and rapid method to verify smoking status among smokers, passive smokers, and nonsmokers by quantification of cotinine in saliva and urine using liquid chromatography and mass spectrometry. Materials and Methods: Cotinine (urine and saliva) levels were measured in 98 participants out of which active users (smoked tobacco users; n = 56) and persons exposed to tobacco smoke (passive smokers; n = 15). Values obtained were compared with nonusers (nonsmokers; n = 27). A simple, rapid, and sensitive method was developed and validated for this purpose. With minimal sample preparation, the current analytical procedure showed a wide detection range (1.1–1000 ng/mL) which made it suitable for analyzing various biological matrices. Results: The mean cotinine levels of urine for smokers, passive smokers, and nonsmokers were 1043.7, 36.63, and 13.6 ng/ml, respectively, while in saliva, it was 327.39, 18.31, and 9.53 ng/ml, respectively. Conclusion: Analysis of variance showed that cotinine levels (urine and saliva) of smokers were significantly higher levels than passive smokers and nonsmokers (P < 0.01). Similarly, passive smokers also had significantly higher cotinine levels (urine and saliva) than nonsmokers (P < 0.001).
Databáze:	OpenAIRE
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