Differential Pharmacological Regulation of Sensorimotor Gating Deficit in CB1 Knockout Mice and Associated Neurochemical and Histological Alterations
| Autor: | Auxiliadora Aracil-Fernández, Pere Berbel, Francisco Navarrete, A. Ortega-Alvaro, Daniela Navarro, Jorge Manzanares |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Fundación Alicia Koplowitz, Instituto de Salud Carlos III |
| Rok vydání: | 2015 |
| Předmět: |
Male
AM251 medicine.medical_specialty Time Factors Cannabinoid receptor Gene Expression Pharmacology Neurochemical Piperidines Receptor Cannabinoid CB1 Receptors Adrenergic alpha-2 Internal medicine medicine Haloperidol Animals Cannabinoid Receptor Antagonists Prepulse inhibition Dopamine transporter Cerebral Cortex Mice Knockout Neurons Dopamine Plasma Membrane Transport Proteins Dose-Response Relationship Drug biology Prepulse Inhibition Methylphenidate business.industry Risperidone Immunohistochemistry Peptide Fragments Psychiatry and Mental health Parvalbumins Endocrinology Knockout mouse biology.protein Pyrazoles Original Article Cholecystokinin business Central Nervous System Agents medicine.drug |
| Zdroj: | Neuropsychopharmacology. 40:2639-2647 |
| ISSN: | 1740-634X 0893-133X |
| DOI: | 10.1038/npp.2015.113 |
| Popis: | The endocannabinoid system has been widely involved in the pathophysiology of sensorimotor gating deficits. This study aimed to evaluate the pharmacological modulation of the sensorimotor gating impairment induced by cannabinoid CB1 receptor (CB1r) deletion. For this purpose, the prepulse inhibition (PPI) paradigm was used to evaluate the effect of two antipsychotics drugs (risperidone and haloperidol) and a psychostimulant (methylphenidate) on the preattentional deficit presented by CB1KO mice. Furthermore, the effects of the CB1r antagonist AM251 on PPI were evaluated in WT mice. Real-time PCR and immunohistochemical studies were carried out to analyze dopamine transporter (DAT) and α-2C adrenergic receptor (ADRA2C) gene expressions and the distribution of parvalbumin (PV) and cholecystokinin-8 (CCK) immunoreactive (ir) cortical neurons, respectively. Neither risperidone nor haloperidol significantly modified the PPI of WT and CB1KO mice, whereas methylphenidate improved the preattentional deficit of CB1KO mice. In addition, treatment with AM251 (3 mg/kg; i.p.) significantly decreased the PPI of WT animals. The administration of methylphenidate increased DAT and ADRA2C gene expressions in CB1KO mice without producing any effect in WT animals. Immunohistochemical studies revealed that there were no significant changes in CCK immunolabeling between WT and CB1KO mice, whereas the radial distribution of PV-ir neurons was abnormal in CB1KO mice. These data further support the important role of CB1r in sensorimotor gating regulation and the therapeutic usefulness of methylphenidate for the treatment of psychiatric disorders with associated preattentional deficits. This study was supported by the following research grants: Ministerio de Ciencia e Innovación SAF2011–23420 to Jorge Manzanares and SAF2009–10689 to Pere Berbel; Foundation Alicia Koplowitz to Pere Berbel; and Instituto de Salud ‘Carlos III’ (FIS), RETICS, Red de Trastornos Adictivos (RD06/0001/1004, RD12/0028/0019) to Jorge Manzanares. |
| Databáze: | OpenAIRE |
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