Quercetin and catechin synergistically inhibit angiotensin II-induced redox-dependent signalling pathways in vascular smooth muscle cells from hypertensive rats

Autor: Andrea Fernanda Gil Lorenzo, Natalia Estrella, Montserrat Cruzado, Claudia Castro, Analia Lourdes Redondo
Rok vydání: 2012
Předmět:
Vascular smooth muscle
medicine.disease_cause
Biochemistry
p38 Mitogen-Activated Protein Kinases
Catechin
Muscle
Smooth
Vascular
chemistry.chemical_compound
Cell Movement
Rats
Inbred SHR
OXIDATIVE STRESS
Phosphorylation
Cells
Cultured
Oxidase test
Membrane Glycoproteins
Chemistry
Angiotensin II
Drug Synergism
General Medicine
Bioquímica y Biología Molecular
Medicina Básica
Hypertension
NADPH Oxidase 2
cardiovascular system
Quercetin
Mitogen-Activated Protein Kinases
Oxidation-Reduction
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
MAP Kinase Signaling System
Internal medicine
QUERCETIN
medicine
Animals
SMOOTH MUSCLE CELLS
Cell Proliferation
HYPERTENSION
ANGIOTENSIN II
Cell growth
NADPH Oxidases
Rats
Oxidative Stress
Endocrinology
CATECHIN
NAD+ kinase
Reactive Oxygen Species
Oxidative stress
Zdroj: Free radical research. 46(5)
ISSN: 1029-2470
Popis: Dietary flavonoids, present in different amount in foods, are associated with the prevention of hypertension, but little is known about the interactions between them. The aim of this study was to explore the effect of quercetin (Q), catechin (C) and the mixture, on Angiotensin II (AngII)-induced redox-dependent signalling pathways and cell behaviour. Mesenteric smooth muscle cells (MesSMC) from spontaneously hypertensive rats (SHR) were incubated with AngII (0.1 μmol/L) alone, or with the mixture of low concentrations of Q and C. AngII-increased ROS production was reduced by the mixture of separately ineffective low concentration of Q (15 μmol/L) plus C (20 μmol/L). This mixture reduced AngII-stimulated NAD(P)H oxidase activation and p47phox translocation to the cell membrane, without affecting Nox2 expression. Co-incubation of Q + C significantly inhibited AngII-induced migration and proliferation, and these effects were independent of p-ERK1/2 and related with reduced p38MAPK phosphorylation. These findings demonstrated that low concentrations of singly non-effective flavonoids when are combined exert a synergistic effect in inhibiting AngII-induced redox-sensitive signalling pathways. Fil: Redondo, Analia Lourdes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Estrella, Natalia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Gil Lorenzo, Andrea Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Cruzado, Montserrat Cecilia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Castro, Claudia Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Databáze: OpenAIRE
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