| Autor: |
Dressler, Franz F., Schoenfeld, Jana, Revyakina, Olga, Vogele, Daniel, Kiefer, Selina, Kirfel, Jutta, Gemoll, Timo, Perner, Sven |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.19695540.v1 |
| Popis: |
Additional file 1: Method S1. Stage-tip protocol. Fig. S1. Exemplary section of an FFPE urothelial cancer specimen. Asterisk marks interdigitating tumor cells within rich desmoplastic stroma, which makes extraction challenging; Hematoxylin-eosin stain, 20× magnification. Fig. S2: Broadened scavenger spectrum by addition of amino acids during antigen retrieval/crosslink reversal. Exemplary western blot showing unaltered extraction efficiency in various buffers (as in Fig. 3). Fig. S3: Extraction performance of the simplified buffer across tissue entities. Coomassie-stained SDS PAGE gel; 1 = pulmonary squamous cell carcinoma; 2 = pulmonary adenocarcinoma; 3/4 = prostate adenocarcinoma specimens; 5/6 = colorectal adenocarcinoma specimens; 40 % of maximum lane volume was loaded (corresponding to 20–40 μg per lane). Table S1. Mass-spectrometric comparison of different sample preparation protocols. X = xylol-based deparaffinization; E = ethanol-based rehydratation; H = heptane-based deparaffinization; M = methanol-based rehydratation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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