An actin-related protein that is most highly expressed in Drosophila testes is critical for embryonic development
| Autor: | Sarah A. Tomlin, John R Valenzuela, Isabel Mejia Natividad, Harmit S. Malik, Janet M. Young, Courtney M. Schroeder |
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| Rok vydání: | 2021 |
| Předmět: |
Male
QH301-705.5 Science Population Embryonic Development General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine evolution Testis Animals Drosophila Proteins Biology (General) Cytoskeleton education development Drosophila Actin 030304 developmental biology fertility 0303 health sciences education.field_of_study D. melanogaster General Immunology and Microbiology biology General Neuroscience Ovary Embryogenesis Genetics and Genomics cytoskeleton Embryo Cell Biology General Medicine biology.organism_classification Actins Cell biology Medicine Female Drosophila melanogaster actin 030217 neurology & neurosurgery Function (biology) Research Article |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| DOI: | 10.7554/elife.71279 |
| Popis: | Most actin-related proteins (Arps) are highly conserved and carry out well-defined cellular functions in eukaryotes. However, many lineages like Drosophila and mammals encode divergent non-canonical Arps whose roles remain unknown. To elucidate the function of non-canonical Arps, we focus on Arp53D, which is highly expressed in testes and retained throughout Drosophila evolution. We show that Arp53D localizes to fusomes and actin cones, two germline-specific actin structures critical for sperm maturation, via a unique N-terminal tail. Surprisingly, we find that male fertility is not impaired upon Arp53D loss, yet population cage experiments reveal that Arp53D is required for optimal fitness in Drosophila melanogaster. To reconcile these findings, we focus on Arp53D function in ovaries and embryos where it is only weakly expressed. We find that under heat stress Arp53D-knockout (KO) females lay embryos with reduced nuclear integrity and lower viability; these defects are further exacerbated in Arp53D-KO embryos. Thus, despite its relatively recent evolution and primarily testis-specific expression, non-canonical Arp53D is required for optimal embryonic development in Drosophila. |
| Databáze: | OpenAIRE |
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