Effects of liquid holding on the induction of mutations in an ultraviolet-sensitive strain of Aspergillus nidulans
| Autor: | R.W. Tuveson, Gisela Wohlrab |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Ultraviolet Rays
Health Toxicology and Mutagenesis Mutant medicine.disease_cause Methionine Aspergillus nidulans Genetics medicine Radiation Genetics Radiosensitivity Purine metabolism Molecular Biology chemistry.chemical_classification Mutation biology Strain (chemistry) Adenine Temperature Water biology.organism_classification Molecular biology Amino acid Aspergillus chemistry Biochemistry Yield (chemistry) |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 8:265-275 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(69)90006-2 |
| Popis: | It has been shown that a mutation to UV-sensitivity ( uvs -I) has the effect of reducing the yield of adenine-independent mutants in Aspergillus nidulans whether the comparison is made on the basis of dose or survival. Methionine-independent mutants were induced with a greater frequency only at higher doses of UV and lower levels of survival. When the uvs -I strain is held at 25° in buffer following UV treatment, survival increases to a maximum at between 48 and 96 h. The level of survival attained by the UV-sensitive mutant during liquid holding is equivalent to that observed with a uvs + strain plated immediately following UV treatment or after 96 h of liquid holding. The uvs -I mutation allows for the expression of liquid-holding repair of UV damage in A. nidulans . The yield of both adenine- or methionine-independent mutants based on survival increased during liquid holding of the uvs -I strain to a level greater than that observed in an equivalent uvs + strain. The yield of adenine- or methionine-independent mutants in the uvs + strain was not altered significantly during liquid holding. We interpret the uvs -I mutant of A. nidulans to represent a regulatory mutation in a function concerned with the repair of radiation damage which in addition to affecting the rate of repair leads to reduced fidelity of repair. |
| Databáze: | OpenAIRE |
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