Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
| Autor: | Ann E. Meyers, Josefina Baztarrica, Vanesa Ruiz, Edward P. Rybicki, Andrés Wigdorovitz |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
NICOTIANA BENTHAMIANA lcsh:Biotechnology viruses medicine.medical_treatment Protein subunit Nicotiana benthamiana FOOT-AND-MOUTH DISEASE VIRUS Applied Microbiology and Biotechnology law.invention 03 medical and health sciences 0302 clinical medicine law VIRUS-LIKE PARTICLES lcsh:TP248.13-248.65 medicine 030212 general & internal medicine Protease biology Ciencias Veterinarias Immunogenicity Antibody titer virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification Virology 030104 developmental biology Capsid CIENCIAS AGRÍCOLAS Recombinant DNA Foot-and-mouth disease virus purl.org/becyt/ford/4.3 [https] TRANSIENT EXPRESSION purl.org/becyt/ford/4 [https] Biotechnology |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Biotechnology Reports, Vol 20, Iss, Pp-(2018) |
| Popis: | Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. Fil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina Fil: Rybicki, Edward P.. University of Cape Town; Sudáfrica Fil: Meyers, Ann E.. University of Cape Town; Sudáfrica Fil: Wigdorovitz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina |
| Databáze: | OpenAIRE |
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