Exogenous γ-aminobutyric acid (GABA) affects pollen tube growth via modulating putative Ca2+-permeable membrane channels and is coupled to negative regulation on glutamate decarboxylase
| Autor: | Ravishankar Palanivelu, Ju You Wu, Jing Feng, Guang Hui Yu, Meng Xiang Sun, Ying Liang Wu, Xiong Bo Peng, Jie Zou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Ca2+-permeable channel Calmodulin Physiology Glutamate decarboxylase Plant Science Pollen Tube medicine.disease_cause Real-Time Polymerase Chain Reaction 01 natural sciences Aminobutyric acid gamma-Aminobutyric acid 03 medical and health sciences Pollen Tobacco otorhinolaryngologic diseases medicine Extracellular γ-Aminobutyric acid gamma-Aminobutyric Acid 030304 developmental biology Plant Proteins 0303 health sciences Voltage-dependent calcium channel biology Nicotiana tabacum Glutamate Decarboxylase Cell Membrane food and beverages Cell biology cell–cell communication Biochemistry biology.protein Pollen tube Calcium Channels pollen tube 010606 plant biology & botany medicine.drug Research Paper Signal Transduction |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Summary This work reveals how tobacco pistil and style may communicate with pollen tubes and regulate their growth during fertilization via the γ-aminobutyric acid–Ca2+-permeable channel–glutamate decarboxylase–calmodulin signalling pathway. γ-Aminobutyric acid (GABA) is implicated in pollen tube growth, but the molecular and cellular mechanisms that it mediates are largely unknown. Here, it is shown that exogenous GABA modulates putative Ca2+-permeable channels on the plasma membranes of tobacco pollen grains and pollen tubes. Whole-cell voltage-clamp experiments and non-invasive micromeasurement technology (NMT) revealed that the influx of Ca2+ increases in pollen tubes in response to exogenous GABA. It is also demonstrated that glutamate decarboxylase (GAD), the rate-limiting enzyme of GABA biosynthesis, is involved in feedback controls of Ca2+-permeable channels to fluctuate intracellular GABA levels and thus modulate pollen tube growth. The findings suggest that GAD activity linked with Ca2+-permeable channels relays an extracellular GABA signal and integrates multiple signal pathways to modulate tobacco pollen tube growth. Thus, the data explain how GABA mediates the communication between the style and the growing pollen tubes. |
| Databáze: | OpenAIRE |
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