Extensive post-transcriptional regulation of microRNAs and its implications for cancer
| Autor: | Scott Hammond, Martin A. Newman, Tricia M. Wright, Elizabeth M. Morin-Kensicki, Joel S. Parker, J. Michael Thomson |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Ribonuclease III
Teratocarcinoma Down-Regulation Embryonic Development Computational biology Cell Line Research Communication Mice Cell Line Tumor Neoplasms microRNA Gene expression Genetics medicine Gene silencing Animals RNA Processing Post-Transcriptional Post-transcriptional regulation Gene Drosha Oligonucleotide Array Sequence Analysis biology Reverse Transcriptase Polymerase Chain Reaction Stem Cells Cancer medicine.disease Blotting Northern Embryo Mammalian Up-Regulation MicroRNAs biology.protein Female Developmental Biology |
| Popis: | MicroRNAs (miRNAs) are short, noncoding RNAs that post-transcriptionally regulate gene expression. While hundreds of mammalian miRNA genes have been identified, little is known about the pathways that regulate the production of active miRNA species. Here we show that a large fraction of miRNA genes are regulated post-transcriptionally. During early mouse development, many miRNA primary transcripts, including the Let-7 family, are present at high levels but are not processed by the enzyme Drosha. An analysis of gene expression in primary tumors indicates that the widespread down-regulation of miRNAs observed in cancer is due to a failure at the Drosha processing step. These data uncover a novel regulatory step in miRNA function and provide a mechanism for miRNA down-regulation in cancer. |
| Databáze: | OpenAIRE |
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