Impact of different nitrogen sources on the growth of Arthrospira sp. PCC 8005 under batch and continuous cultivation – A biochemical, transcriptomic and proteomic profile
| Autor: | Orily Depraetere, Ruddy Wattiez, Frédéric Deschoenmaeker, Juan Carlos Cabrera Pino, Koenraad Muylaert, Guillaume Bayon-Vicente, Neha Sachdeva, Baptiste Leroy |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Cyanobacteria Environmental Engineering Nitrogen 030106 microbiology Photobioreactor Bioengineering 03 medical and health sciences chemistry.chemical_compound Nitrate Spirulina Bioreactor Ammonium Waste Management and Disposal Nitrogen cycle Nitrates biology Renewable Energy Sustainability and the Environment Gene Expression Regulation Bacterial General Medicine biology.organism_classification 030104 developmental biology chemistry Biochemistry Urea Arthrospira |
| Zdroj: | Bioresource Technology. 237:78-88 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2017.03.145 |
| Popis: | The aim of the present study was to evaluate the effects of varying concentrations of different nitrogen sources (individually or in combination) on the biochemical, transcriptomic and proteomic profiles of Arthrospira sp. PCC 8005 under batch and continuous modes. In batch mode, while ammonium showed a repressive effect on nitrate-assimilation pathway of the cyanobacteria; better growth and nutrient uptake rate were observed in presence of urea than nitrate. The inhibitory effect of ammonium was further confirmed by the continuous photobioreactor study wherein the nutrient feed was transiently replaced from nitrate to ammonium (28mM turbiostat regime). The changes in lipid, exopolysaccharide, transcriptomic and proteomic profiles of cyanobacteria on transition from nitrate to ammonium indicated at an onset of nutrient stress. |
| Databáze: | OpenAIRE |
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