Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment
| Autor: | Chi-Yong Ahn, Youlboong Sung, Kyung-Hwa Baek, Hee-Mock Oh, Hee-Sik Kim, Dae-Hyun Cho, Sung-Cheol Koh, Byung-Hyuk Kim |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Geologic Sediments Tetrachloroethylene Time Factors Ethylene Bioengineering Electron donor DNA Ribosomal Applied Microbiology and Biotechnology Enrichment culture Microbiology Geobacter lovleyi chemistry.chemical_compound RNA Ribosomal 16S Reductive dechlorination Anaerobiosis Lactic Acid Dehalococcoides Bacteria biology Chemistry Sequence Analysis DNA General Medicine Ethylenes biology.organism_classification Microbial population biology Environmental chemistry Chlorine Oxidation-Reduction Anaerobic exercise Biotechnology |
| Zdroj: | Biotechnology Letters. 32:1829-1835 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-010-0381-y |
| Popis: | A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 μmol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P. |
| Databáze: | OpenAIRE |
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