Flavonoid-rich fraction attenuates permethrin-induced toxicity by modulating ROS-mediated hepatic oxidative stress and mitochondrial dysfunction ex vivo and in vivo in rat
| Autor: | Manel Naifar, Hajer Jdidi, F. Makni, Fatma Ghorbel Koubaa, Hamadi Fetoui, Houda Ayadi, Sami Maalej, Abdelfattah El Feki, Nissaf Aoiadni |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Health Toxicology and Mutagenesis medicine.medical_treatment 010501 environmental sciences Pharmacology Protein oxidation medicine.disease_cause 01 natural sciences Antioxidants Lipid peroxidation chemistry.chemical_compound In vivo medicine TBARS Animals Environmental Chemistry Permethrin 0105 earth and related environmental sciences Flavonoids Plant Extracts General Medicine Pollution Mitochondria Rats Oxidative Stress Liver chemistry Myricetin Chemical and Drug Induced Liver Injury Reactive Oxygen Species Ex vivo Oxidative stress |
| Zdroj: | Environmental Science and Pollution Research. 28:9290-9312 |
| ISSN: | 1614-7499 0944-1344 |
| DOI: | 10.1007/s11356-020-11250-9 |
| Popis: | The present study explores the antioxidant, anti-microbial, and hepatoprotective potentials of flavonoid-rich fractions from Fumaria officinalis against permethrin-induced liver damage ex vivo/in vivo in rat. However, HPLC-DAD analysis revealed the richness of 6 components in ethyl acetate fraction (EAF) where ferulic acid, rosmarinic acid, and myricetin are the most abundant. The in vitro assays showed that EAFs have impressive antioxidant and anti-microbial properties. Ex vivo, permethrin (PER) (100 μM) induced a decrease of hepatic AST and ALT activities and 25-OH vitamin D and vitamin C levels and an increase of ALP and LDH activities, TBARS, and ϒ-GT levels with a disturbance of oxidative status. The hepatoprotective effect of EAF (1 mg/mL) against PER was confirmed by the amelioration of oxidative stress profile. In vivo, permethrin was found to increase absolute and relative liver weights, plasma transaminase activities, lactate-to-pyruvate ratio, hepatic and mitochondrial lipid peroxidation, and protein oxidation levels. This pesticide triggered a decrease of Ca2+ and Mg2+-ATPases and mitochondrial enzyme activities. The co-treatment with EAF reestablished the hepatic and mitochondrial function, which could be attributed to its richness in phenolic compounds. |
| Databáze: | OpenAIRE |
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