Molecular epidemiology of respiratory syncytial virus in children in sub-Saharan Africa

Autor: Paul Schnitzler, José Francisco Fernandes, Daniel Eibach, Juergen May, Norbert Heinrich, Andiyam Thierry Ouédraogo, Sabine Gies, Jana Held, Benjamin Mordmüller, Chacha Mangu, Ali Sié, Isabella Eckerle, Clara Marlene Ihling, Julia Tabatabai, Boubacar Coulibaly, Benedikt Hogan, Lwitiho Sudi, Ayola A. Adegnika, Aurélia Souares
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Glycosylation
viruses
medicine.disease_cause
Polymerase Chain Reaction
Ghana
Tanzania
0302 clinical medicine
Polymerase Chain Reaction / methods
Genotype
Children
Phylogeny
ddc:616
Molecular Epidemiology
Phylogenetic tree
virus diseases
RSV
respiratory system
Sequence Analysis
DNA / methods
Infectious Diseases
GenBank
Child
Preschool
Molecular epidemiology
Respiratory Syncytial Virus Infections / diagnosis
Female
Rhinovirus
Molecular Epidemiology / methods
030231 tropical medicine
Genotypes
Respiratory Syncytial Virus Infections
Biology
Virus
03 medical and health sciences
parasitic diseases
Burkina Faso
medicine
Humans
Gabon
Africa South of the Sahara
Public Health
Environmental and Occupational Health
Infant
Sequence Analysis
DNA
biology.organism_classification
Respiratory Syncytial Virus
Human / genetics
Virology
Hypervariable region
Respiratory Syncytial Virus
Human
Respiratory Syncytial Virus Infections / genetics
Africa
Parasitology
Zdroj: Tropical medicine & international health, Vol. 26, No 7 (2021) pp. 810-822
ISSN: 2011-2017
1360-2276
Popis: Objectives This study investigated the molecular epidemiology of respiratory syncytial virus (RSV) among febrile children with acute respiratory tract infection in Ghana, Gabon, Tanzania and Burkina Faso between 2014 and 2017 as well as the evolution and diversification of RSV strains from other sub-Saharan countries. Methods Pharyngeal swabs were collected at four study sites (Agogo, Ghana: n = 490; Lambarene, Gabon: n = 182; Mbeya, Tanzania: n = 293; Nouna, Burkina Faso: n = 115) and analysed for RSV and other respiratory viruses using rtPCR. For RSV-positive samples, sequence analysis of the second hypervariable region of the G gene was performed. A dataset of RSV strains from sub-Saharan Africa (2011-2017) currently available in GenBank was compiled. Phylogenetic analysis was conducted to identify the diversity of circulating RSV genotypes. Results In total, 46 samples were tested RSV positive (Ghana n = 31 (6.3%), Gabon n = 4 (2.2%), Tanzania n = 9 (3.1%) and Burkina Faso n = 2 (1.7%)). The most common RSV co-infection was with rhinovirus. All RSV A strains clustered with genotype ON1 strains with a 72-nucleotide duplication and all RSV B strains belonged to genotype BAIX. Phylogenetic analysis of amino acid sequences from sub-Saharan Africa revealed the diversification into 11 different ON1 and 22 different BAIX lineages and differentiation of ON1 and BAIX strains into potential new sub-genotypes, provisionally named ON1-NGR, BAIX-KEN1, BAIX-KEN2 and BAIX-KEN3. Conclusion The study contributes to an improved understanding of the molecular epidemiology of RSV infection in sub-Saharan Africa. It provides the first phylogenetic data for RSV from Tanzania, Gabon and Burkina Faso and combines it with RSV strains from all other sub-Saharan countries currently available in GenBank.
Databáze: OpenAIRE
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