Immunohistochemical and Molecular Analysis of Human Melanomas for Expression of the Human Cancer-Testis Antigens NY-ESO-1 and LAGE-1
| Autor: | Ian D. Davis, Achim A. Jungbluth, Judy Browning, Jonathan Cebon, Sue Sturrock, Philip Parente, Yao-Tseng Chen, Suzanne Svobodova, Elisabeth Stockert, Fiona St. Clair, Lloyd J. Old, Hilary A. Vaughan, Duncan MacGregor |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Cancer Research Skin Neoplasms medicine.drug_class Monoclonal antibody Immunoenzyme Techniques Antigen Antigens Neoplasm Testis medicine Humans Tissue Distribution Typing Melanoma Polymorphism Genetic biology Reverse Transcriptase Polymerase Chain Reaction Antibodies Monoclonal Membrane Proteins Molecular biology Gene Expression Regulation Neoplastic Oncology Antigens Surface Monoclonal biology.protein Cancer/testis antigens Immunohistochemistry Antibody NY-ESO-1 |
| Zdroj: | Clinical Cancer Research. 10:8396-8404 |
| ISSN: | 1557-3265 1078-0432 |
| Popis: | Purpose: NY-ESO-1 and LAGE-1 are homologous cancer-testis antigens, which are expressed in many different cancers. It is essential to type tumors accurately to assess patient suitability for clinical trials which target these. This study evaluates typing strategies used to distinguish these two homologous but distinct antigens and to characterize and quantitate expression of each in clinical samples. Experimental Design: We typed 120 malignant melanomas for the expression of NY-ESO-1 and LAGE-1 with immunohistochemistry, reverse transcription-PCR (RT-PCR), and quantitative real-time (qRT-PCR), which was also used to explore the relationship between NY-ESO-1 and LAGE expression. Results: The two monoclonal antibodies ES121 and E978 had very similar immunohistochemistry reactivities. Both were specific for NY-ESO-1 because neither bound to homologous LAGE-1 peptides despite 84% overall amino acid homology. Of 120 melanomas tested by immunohistochemistry, NY-ESO-1 was expressed in >50% of cells in 23 melanomas (19%), between 11 and 50% cells in 15 (12.5%), Conclusions: For NY-ESO-1 typing, immunohistochemistry compared favorably with the RT-PCR, with the added advantage of being able to characterize heterogeneity of antigen expression. Because neither mAb bound LAGE and because there was no coordinate expression LAGE and NY-ESO-1, separate typing for each is required. |
| Databáze: | OpenAIRE |
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