Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes
| Autor: | M Zoran Vujcic, I Anica Horvat, M Vesna Vasic, Tatjana Momić |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
mercury
cadmium Metal ions in aqueous solution chemistry.chemical_element 01 natural sciences lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Adsorption Na+/K+-ATPase 030304 developmental biology chemistry.chemical_classification 0303 health sciences Cadmium Chromatography biology 010405 organic chemistry synaptic plasma membranes fungi na k-atpase General Chemistry Enzyme assay inhibition 0104 chemical sciences Membrane Enzyme lcsh:QD1-999 chemistry Na K-ATPase immobilization biology.protein Nitrocellulose |
| Zdroj: | Journal of the Serbian Chemical Society Journal of the Serbian Chemical Society, Vol 67, Iss 12, Pp 809-817 (2002) |
| Popis: | Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma mem- branes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluo- ride (PVDF) and glass fiber (GF) membranes. Partial SDS solubilization increased the en- zyme activity by 40 %. With regard to the preservation of the enzyme activity, nitrocellulose was shown to be the optimal support for the immobilization. The enzyme showed the high- est percentage activity (14 %) after 30 min of SPM adsorption, at 20 oC under the vaccum, with 25 g of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 oC. After adsorp- tion, the sensitivity of the enzyme to HgCl 2 and CdCl 2 inhibition was higher. The results show that immobilized Na,K-ATPase SPM can be used as a practical model for the detection of metal ions in different samples. |
| Databáze: | OpenAIRE |
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