Efficient production of antibody fragments by the filamentous fungus trichoderma reesei
| Autor: | Jonathan Knowles, Anu Marjukka Harkki, Sirkka Keränen, Eini Nyyssönen, Anu Saloheimo, Merja Penttilä |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Recombinant Fusion Proteins
Trichoderma longibrachiatum medicine.medical_treatment Blotting Western Genetic Vectors Molecular Sequence Data Biomedical Engineering Bioengineering Cellulase Transfection Applied Microbiology and Biotechnology Microbiology law.invention Immunoglobulin Fab Fragments Mice Antigen law Endopeptidases medicine Animals Amino Acid Sequence Trichoderma reesei Trichoderma Gel electrophoresis Protease Base Sequence biology Oxazolone biology.organism_classification Fusion protein Immunoglobulin G biology.protein Recombinant DNA Molecular Medicine Genetic Engineering Plasmids Biotechnology |
| Zdroj: | Nyyssönen, E, Penttilä, M, Harkki, A, Saloheimo, A, Knowles, J K C & Keränen, S 1993, ' Efficient production of antibody fragments by the filamentous fungus trichoderma reesei ', Bio/Technology, vol. 11, no. 5, pp. 591-595 . https://doi.org/10.1038/nbt0593-591 |
| DOI: | 10.1038/nbt0593-591 |
| Popis: | We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|