SPR-5 is a histone H3K4 demethylase with a role in meiotic double-strand break repair
| Autor: | Amanda C. Nottke, Valerie Reinke, Sara E. Beese-Sims, Luiz F. Pantalena, Yang Shi, Monica P. Colaiácovo |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
DNA Repair
DNA repair education Blotting Western Green Fluorescent Proteins Apoptosis Biology Methylation Animals Genetically Modified Histones RNA interference Transcription (biology) Histone methylation Animals DNA Breaks Double-Stranded Caenorhabditis elegans Caenorhabditis elegans Proteins Oligonucleotide Array Sequence Analysis Genetics Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Lysine Oxidoreductases N-Demethylating Biological Sciences biology.organism_classification Antineoplastic Agents Phytogenic Double Strand Break Repair Meiosis Histone Germ Cells Microscopy Fluorescence Mutation biology.protein Demethylase Camptothecin RNA Interference Rad51 Recombinase |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 108(31) |
| ISSN: | 1091-6490 |
| Popis: | Regulation of histone methylation levels has long been implicated in multiple cellular processes, many of which involve transcription. Here, however, we report a unique role for the Caenorhabditis elegans histone demethylase SPR-5 in meiotic DNA double-strand break repair (DSBR). SPR-5 shows enzymatic activity toward H3K4me2 both in vitro and in the nematode germline, and spr-5 mutants show several phenotypes indicating a perturbation of DSBR, including increased p53-dependent germ cell apoptosis, increased levels of the DSBR marker RAD-51, and sensitivity toward DSB-inducing treatments. spr -5 mutants show no transcriptional misregulation of known DSBR involved genes. Instead, SPR-5 shows a rapid subcellular relocalization upon DSB-inducing treatment, which suggests that SPR-5 may function directly in DSBR. |
| Databáze: | OpenAIRE |
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