MAIT cell alterations in adults with recent-onset and long-term type 1 diabetes

Autor: Christian Boitard, Léo Bertrand, Camille Rousseau, Pauline Soulard, Isabelle Nel, Etienne Larger, Agnès Lehuen, Matthieu Rouland, Lucie Beaudoin, Zouriatou Gouda
Přispěvatelé: Institut Cochin, Inserm, CNRS, Laboratory of Excellence Inflamex, Université de Paris, Paris, France, Diabetology Department, Cochin Hospital, AP-HP Centre - Université de Paris, Paris, France, ROULAND, Matthieu
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Antigens
Differentiation
T-Lymphocyte
Male
Granzyme B production
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Programmed Cell Death 1 Receptor
Blood Donors
Autoimmunity
medicine.disease_cause
0302 clinical medicine
Glucose homeostasis
IL-2 receptor
ComputingMilieux_MISCELLANEOUS
Innate immunity
0303 health sciences
Middle Aged
Flow Cytometry
3. Good health
[SDV] Life Sciences [q-bio]
Phenotype
Type 1 diabetes
Cytokine
Proto-Oncogene Proteins c-bcl-2
Cytokines
Female
Human
Adult
MAIT cells
Mucosal-Associated Invariant T Cells
Article
Young Adult
03 medical and health sciences
Antigens
CD
Internal Medicine
medicine
Humans
Lectins
C-Type
Interleukin-7 receptor
Aged
030304 developmental biology
Granzyme
Autoimmune disease
business.industry
Interleukin-2 Receptor alpha Subunit
medicine.disease
Diabetes Mellitus
Type 1
Immunology
business
Biomarkers
030215 immunology
Zdroj: Diabetologia
Diabetologia, Springer Verlag, 2021, 64 (10), pp.2306-2321. ⟨10.1007/s00125-021-05527-y⟩
ISSN: 0012-186X
1432-0428
Popis: Aims/hypothesis Mucosal-associated invariant T (MAIT) cells are innate-like T lymphocytes expressing an αβ T cell antigen receptor that recognises the MHC-related 1 molecule. MAIT cells are altered in children at risk for and with type 1 diabetes, and mouse model studies have shown MAIT cell involvement in type 1 diabetes development. Since several studies support heterogeneity in type 1 diabetes physiopathology according to the age of individuals, we investigated whether MAIT cells were altered in adults with type 1 diabetes. Methods MAIT cell frequency, phenotype and function were analysed by flow cytometry, using fresh peripheral blood from 21 adults with recent-onset type 1 diabetes (2–14 days after disease onset) and 47 adults with long-term disease (>2 years after diagnosis) compared with 55 healthy blood donors. We also separately analysed 17 women with long-term type 1 diabetes and an associated autoimmune disease, compared with 30 healthy women and 27 women with long-term type 1 diabetes. Results MAIT cells from adults with recent-onset type 1 diabetes, compared with healthy adult donors, harboured a strongly activated phenotype indicated by an elevated CD25+ MAIT cell frequency. In adults with long-term type 1 diabetes, MAIT cells displayed an activated and exhausted phenotype characterised by high CD25 and programmed cell death 1 (PD1) expression and a decreased production of proinflammatory cytokines, IL-2, IFN-γ and TNF-α. Even though MAIT cells from these patients showed upregulated IL-17 and IL-4 production, the polyfunctionality of MAIT cells was decreased (median 4.8 vs 13.14% of MAIT cells, p
Databáze: OpenAIRE
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