The UGA-CiE1 cell line from Chrysodeixis includens exhibits characteristics of granulocytes and is permissive to infection by two viruses
| Autor: | Kavita Bitra, Shu Zhang, Jena A. Johnson, Lihua Wang, Dwight E Lynn, Michael R. Strand |
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| Rok vydání: | 2010 |
| Předmět: |
Hemocytes
food.ingredient Genes Viral Moths Transfection Chrysodeixis Biochemistry Virus Cell Line food Chrysodeixis includens Animals Molecular Biology biology Polydnavirus fungi biology.organism_classification Virology Nucleopolyhedroviruses Autographa californica Cell culture Pseudoplusia Insect Science RNA Interference Bracovirus Granulocytes |
| Zdroj: | Insect Biochemistry and Molecular Biology. 40:394-404 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2010.03.005 |
| Popis: | The soybean looper, Chrysodeixis (Pseudoplusia) includens (Lepidoptera: Noctuidae) is an economically important insect pest and a highly permissive host for the parasitoid Microplitis demolitor and its associated polydnavirus M. demolitor bracovirus (MdBV). Here we established a cell line from C. includens embryos designated UGA-CiE1 cells. CiE1 cells morphologically resemble granulocytes, which are a subpopulation of C. includens hemocytes. Antibody and RT-PCR analyses indicated that CiE1 cells express several molecular and functional markers that identify granulocytes. We further determined that CiE1 cells are permissive to infection by MdBV, exhibiting alterations very similar to MdBV-infected granulocytes, and Autographa californica multiple nucleopolyhedrosis virus (AcMNPV). Combined with the ability to transfect CiE1 cells with high efficiency and knock down expression of viral genes by RNA interference, we conclude this cell line has several attributes of value for studying immune interactions with polydnaviruses and potentially other pathogens. |
| Databáze: | OpenAIRE |
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