A novel point-of-care test of respiratory syncytial viral RNA based on cellulose-based purification and convective PCR
| Autor: | Shiyin Zhang, Ya Zhang, Ningshao Xia, Xuedong Lu, Zhongfu Chen, Jun Zhang, Yixin Chen, Yinhui Zhang, Tingdong Li, Junyu Si, Jin Wang, Shengxiang Ge, Yong-Peng Sun |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Point-of-care testing Clinical Biochemistry Respiratory Syncytial Virus Infections Polymerase Chain Reaction Sensitivity and Specificity Biochemistry Rapid detection Virus law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Nasopharynx Humans Viral rna Cellulose Polymerase chain reaction Chemistry Biochemistry (medical) General Medicine Virology 030104 developmental biology Point-of-Care Testing Respiratory Syncytial Virus Human 030220 oncology & carcinogenesis Nucleic acid RNA Viral Extraction methods |
| Zdroj: | Clinica Chimica Acta. 511:154-159 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/j.cca.2020.10.012 |
| Popis: | Background Respiratory syncytial virus (RSV) infection is a global public-health problem. Timely diagnostics are needed for high-risk patients. Several methods have been used for RSV detection but not suitable for on-site detection due to the requirement of specialized laboratories and expensive equipment. Methods We developed a convenient, rapid and low-cost method of nucleic acids (NA) extraction based on cellulose paper, which could extract NA from nasopharyngeal swabs (NPSs) within 1 min. This extraction method was integrated with fluorescence convection polymerase chain reaction (CPCR), which easily affordable and easy-to-use NA detection of the RSV in 33 min. Results The developed cellulose-based NA purification combine with CPCR (CP-CPCR) reliably detected as little as 0.01 TCID50/mL of RSV cultures. CP-CPCR performance was tested further using NPSs: it showed sensitivity of 100% and a specificity of 100% compared with traditional extraction and amplification methods. Conclusions Our evaluation confirmed high specificity, sensitivity and efficient of the CP-CPCR, which can be used widely for RSV testing in resource-limited settings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect