INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

Autor: Rob J. Rentenaar, Nard G Janssen, Theo van den Broek, David J. Hetem, Jakko van Ingen, Wouter Bekers, Ad C. Fluit, Johannes G. Kusters, Miranda Kamst
Rok vydání: 2019
Předmět:
Adult
Male
Microbiology (medical)
HSP65 sequencing
Sequence analysis
Inno lipa
Mycobacterium smegmatis
Mycobacterium Infections
Nontuberculous
INNO-LiPA
Microbial Sensitivity Tests
Microbiology
chemistry.chemical_compound
All institutes and research themes of the Radboud University Medical Center
Lymphadenitis
Case report
Animals
Humans
Mycobacterium fortuitum complex
Diagnostic Errors
Line Probe Assay
biology
Mycobacterium fortuitum
Chemistry
General Medicine
biology.organism_classification
Mycobacterium fortuitum Complex
Matrix-assisted laser desorption/ionization
Treatment Outcome
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Infectious Diseases
Molecular Diagnostic Techniques
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Pus specimen
Cattle
Reagent Kits
Diagnostic
DNA
Zdroj: Diagnostic Microbiology and Infectious Diseases, 95, 3
Diagnostic Microbiology and Infectious Diseases, 95
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2019.06.010
Popis: Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.
Databáze: OpenAIRE
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