Advantages of a 21-loci short tandem repeat method for detection of cross-contamination in human cell lines
| Autor: | Jingxuan Liu, Yundian Zeng, Chuguang Chen, Suling Duan, Jian Yang, Jie Liu, Xuxu Ding, Chao Shen, Meimei Yang, Meijia Gu, Mingmin Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genetic Markers General Medicine Computational biology Biology Human cell Cell Line Molecular Typing 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Cell Line Authentication Genetic Loci 030220 oncology & carcinogenesis Cell Line Tumor Genetics Microsatellite Humans Microsatellite Repeats |
| Zdroj: | Gene. 763 |
| ISSN: | 1879-0038 |
| Popis: | Cross-contamination of cell lines is a highly relevant and pervasive problem. The analysis of short tandem repeats (STR) is a simple and commercially available technique to authenticate cell lines for more than two decades. At present, STR multiple amplification kits have been developed up to 21 loci while the current STR databases only provide 9-loci STR profiles. Here, we compared the advantages of 21-loci STR methodology using the same algorithm as 9-loci method. The 21-loci method reduced the uncertainty ratio for authentications by 97.5% relative to the 9-loci method and exclude effectively false positive. We show that the additional 12 loci helped to greatly reduce sample-site marker specificity arising from genetic isolation and the occurrence of null alleles, suggesting that inclusion of additional loci in these databases will ultimately improve the efficiency and accuracy of authentication of cell lines. Taken together, we demonstrate the utility of a 21-loci method in human cells, providing a novel marker panel for use as a valuable alternative to 9-loci analyses to minimize cell line authentication errors and reduce costs due to erroneous experiments. |
| Databáze: | OpenAIRE |
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